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Purified Mouse Anti-Mouse Nedd4
Purified Mouse Anti-Mouse Nedd4

Western blot analysis of Nedd4 on a BC3H1 cell lysate (murine skeletal muscle cells). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Nedd4 antibody.

Purified Mouse Anti-Mouse Nedd4

Immunoflourescence staining of RSV-3T3 cells.

Western blot analysis of Nedd4 on a BC3H1 cell lysate (murine skeletal muscle cells). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Nedd4 antibody.

Immunoflourescence staining of RSV-3T3 cells.

Product Details
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BD Transduction Laboratories™
Mouse (QC Testing)
Mouse IgG1
Mouse Nedd4 aa. 173-262
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
110 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611480 Rev. 2
Antibody Details
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Regulated protein degradation involves conjugation of ubiquitin to proteins for degradation by the 26S proteasome. Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1-E3. Neuronal precursor cell-expressed developmentally down-regulated 4 (Nedd4) is a multimodular ubiquitin protein ligase E3 that contains a Ca 2+-lipid binding domain (CaLB), 3 WW domains, and a C-terminal ubiquitin protein ligase hect domain. The CaLB is characteristic of C2 domain-containing proteins, such as PKC, phospholipase C, phospholipase A2, and synaptogamin. Nedd4 is expressed differentially in the fetal and adult brain and its highest expression is in lung and kidney. Although it localizes in the cytoplasm, increased intracellular Ca 2+ induces plasma membrane association of Nedd4. In addition, increases in intracellular Na+ leads to Nedd4 binding to epithelial Na+ channels via its WW domains, which results in targeting of Na+ channels for degradation. Thus, the subcellular localization and protein interactions of Nedd4 are regulated in a Ca2+- and Na+-dependent manner.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611480 Rev. 2
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
611480 Rev.2
Citations & References
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Development References (4)

  1. Harvey KF, Dinudom A, Komwatana P, et al. All three WW domains of murine Nedd4 are involved in the regulation of epithelial sodium channels by intracellular Na+.. J Biol Chem. 1999; 274(18):12525-30. (Biology). View Reference
  2. Hatakeyama S, Jensen JP, Weissman AM. Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases. J Biol Chem. 1997; 272(24):15085-15092. (Biology). View Reference
  3. Peruzzi F, Prisco M, Morrione A, Valentinis B, Baserga R. Anti-apoptotic signaling of the insulin-like growth factor-I receptor through mitochondrial translocation of c-Raf and Nedd4. J Biol Chem. 2001; 276(28):25990-25996. (Biology: Western blot). View Reference
  4. Plant PJ, Yeger H, Staub O, Howard P, Rotin D. The C2 domain of the ubiquitin protein ligase Nedd4 mediates Ca2+-dependent plasma membrane localization. J Biol Chem. 1997; 272(51):32329-32336. (Biology). View Reference
View All (4) View Less
611480 Rev. 2

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