Purified Mouse Anti-Dynamin II

BD Transduction Laboratories™ Purified Mouse Anti-Dynamin II

Name: Purified Mouse Anti-Dynamin II
Brand: Purified Mouse Anti-Dynamin II
SKU: 610263

Clone 27/Dynamin II (RUO)

Western blot analysis of Dynamin II on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2) using 1 µg/mL of the Mouse Anti-Dynamin II antibody.

Immunohistochemical staining for Dynamin II on a rat brain section, zinc-fixed paraffin-embedded (40X magnification).

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Catalog No:610263

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Product Details

Brand: BD Transduction Laboratories™

Reactivity: Human (QC Testing), Mouse, Rat (Tested in Development)

Isotype: Mouse IgG2a

Immunogen: Rat Dynamin II aa. 274-555

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)

Target Molecular Weight: 100 kDa

Concentration: 250 µg/ml

RRID: AB_397659

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Data Sheets

Technical Data Sheet (TDS)

Safety Data Sheet

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Size 1 ML Cat No. 554002

610263 Rev. 2

Antibody Details

27/Dynamin II

Dynamin is a membrane-associated GTPase that binds to GTP, microtubules, and phospholipids. Northern blot analysis has shown dynamin to be present in most tissues but at an amount about 20-fold lower than that in brain. There are at least two distinct dynamin genes in mammals. Transcripts of both dynamin genes are alternatively spliced at two or more sites. The first site is identical in both dynamins, whereas, the second site differs. The two gene products are known as Dynamin I and Dynamin II and show 79% identity. Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous. The two proteins are highly homologous in the N-terminal region, while the C-terminal domain shows significant divergence. The GTPase activity of Dynamin I is stimulated several fold by binding to microtubules, phospholipids, and membranous vesicles. Dynamin I is a good substrate of PKC in vitro as well as in vivo in resting nerve terminals. In vitro, phosphorylation occurs at the C-terminus of Dynamin I and this enhances the GTPase activity more than 10-fold. However, Dynamin II is not a substrate of PKC and its activity does not appear to be affected by phosphorylation. These data suggest that the function of the common N-terminal domain between Dynamin I and II may be differentially specified by distinct C-terminal domains.

610263 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

610263 Rev.2

Citations & References

View Development References

Development References (5)

Cook TA, Urrutia R, McNiven MA. Identification of dynamin 2, an isoform ubiquitously expressed in rat tissues. Proc Natl Acad Sci U S A. 1994; 91(2):644-648. (Biology). View Reference
Kessels MM, Engqvist-Goldstein AE, Drubin DG. Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation. Mol Biol Cell. 2000; 11(1):393-412. (Biology: Immunofluorescence). View Reference
Kranenburg O, Verlaan I, Moolenaar WH. Dynamin is required for the activation of mitogen-activated protein (MAP) kinase by MAP kinase kinase. J Biol Chem. 1999; 274(50):35301-35304. (Biology: Western blot). View Reference
McNiven MA, Kim L, Krueger EW, Orth JD, Cao H, Wong TW. Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shape. J Cell Biol. 2000; 151(1):187-198. (Biology: Immunoprecipitation, Western blot). View Reference
Sontag JM, Fykse EM, Ushkaryov Y, Liu JP, Robinson PJ, Sudhof TC. Differential expression and regulation of multiple dynamins. J Biol Chem. 1994; 269(6):4547-4554. (Biology). View Reference

View All (5)

610263 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.