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APC Mouse anti-Human CD212
APC Mouse anti-Human CD212

Profile of human CD212 (IL-12 receptor β1) expression on stimulated peripheral blood lymphocytes.  Human blood cells were stimulated with CD3/CD28 for three days and then stained with APC-anti CD212 (solid line) or APC mouse IgG1 isotype control (dashed line, Cat. No. 554681).   Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Profile of human CD212 (IL-12 receptor β1) expression on stimulated peripheral blood lymphocytes.  Human blood cells were stimulated with CD3/CD28 for three days and then stained with APC-anti CD212 (solid line) or APC mouse IgG1 isotype control (dashed line, Cat. No. 554681).   Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Product Details
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BD Pharmingen™
IL-12R beta 1; IL-12R-BETA1; IL-12 receptor β1; IMD30
Human (QC Testing)
Mouse IgG1, κ
PHA-activated Human PBMC
Flow cytometry (Routinely Tested)
Optimal
20 µl
VII 70503
3594
AB_1645455
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
558708 Rev. 1
Antibody Details
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2.4E6

The 2.4E6 monoclonal antibody specifically binds to CD212, which is also known as the Interleukin-12 Receptor beta-1 chain (IL12RB1/IL-12RB1/IL-12Rβ1). CD212 serves as a subunit for the functional IL-12 Receptor (IL-12Rβ1-IL-12Rβ2) and IL-23 Receptor (IL-12Rβ1-IL-23R) complexes. CD212 is expressed on T cells, B cells, or NK cells. CD212 is a type I transmembrane glycoprotein that belongs to the hemopoietin receptor superfamily. CD212 is responsible for binding to the IL-12 p40 subunit that is common to the heterodimeric IL-12 and IL-23 cytokines. IL-12 and IL-23 are immunomodulatory cytokines produced mainly by antigen-presenting cells (APC) including monocytes and dendritic cells. These cytokines have overlapping as well as distinct biological activities.

558708 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
APC
Red 627-640 nm
651 nm
660 nm
558708 Rev.1
Citations & References
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Development References (4)

  1. Chua AO, Chizzonite R, Desai BB, et al. Expression cloning of a human IL-12 receptor component. A new member of the cytokine receptor superfamily with strong homology to gp130. J Immunol. 1994; 153(1):128-136. (Immunogen). View Reference
  2. Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
  3. Presky DH, Minetti LJ, Gillessen S, et al. Analysis of the multiple interactions between IL-12 and the high affinity IL-12 receptor complex. J Immunol. 1998; 160(5):2174-2179. (Biology). View Reference
  4. Wu CY, Warrier RR, Carvajal DM, et al. Biological function and distribution of human interleukin-12 receptor beta chain. Eur J Immunol. 1996; 26(2):345-350. (Biology). View Reference
View All (4) View Less
558708 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.