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PE Rat Anti-Mouse IgG2a+b
Product Details
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BD™
Mouse
Rat IgG1
MOPC 141 mouse myeloma protein
Flow cytometry
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The PE and PerCP conjugates are supplied in 2.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340269 Rev. 1
Antibody Details
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X57

Rat Anti-Mouse IgG2a+b, clone X57, is derived from hybridization of rat YB2/0 myeloma cells with spleen cells from LOU/M rats immunized with MOPC 141 mouse myeloma protein. (MOPC 141 is composed of mouse IgG2b heavy chains and kappa light chains.)

340269 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340269 Rev.1
Citations & References
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340269 Rev. 1

 

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.