Description
BD Tritest™ Control, γfluorescein isothiocyanate (FITC)/γphycoerythrin (PE)/CD45 peridinin chlorophyll (PerCP), is a three-color direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with BD Tritest reagents on the BD family of flow cytometers. The BD Tritest Control is used to determine the unstained lymphocyte population on a fluorescence 1 (FL1) vs fluorescence 2 (FL2) display and to determine any nonantigen-specific antibody binding (nonspecific staining) present, particularly that caused by Fc receptors. BD Tritest Control is for in vitro diagnostic use with BD in vitro diagnostic BD Tritest reagents.
Preparation And Storage
• For in vitro diagnostic use.
• Store the reagent at 2°C–8°C to maintain stability. Do not use after expiration date shown on the label.
• Do not freeze the reagent or expose it to direct light during storage or during incubation with cells. Keep the reagent vial dry.
• Do not use the reagent if you observe any changes in appearance. Precipitation or discoloration indicates instability or deterioration.
• The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.
• All biological specimens and materials coming into contact with them are considered biohazards. Handle as if capable of transmitting infection and dispose of with proper precautions in accordance with federal, state, and local regulations when disposing of all materials. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves.
• BD FACS lysing solution is required and contains diethylene glycol and formaldehyde. Refer to the BD FACS Lysing Solution instructions for use (IFU) for warnings.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| CD45 PerCP | 2D1 | IgG1, κ | N/A |
| Mouse IgG1 Isotype Control FITC | X40 | IgG1, κ | N/A |
| Mouse IgG1 Isotype Control PE | L293 | IgG1, κ | N/A |
Development References (11)
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Centers for Disease Control. Revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR. 1997; 46:1-29. (Biology).
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Clinical and Laboratory Standards Institute. 2007. (Biology).
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Clinical and Laboratory Standards Institute. 2007. (Biology).
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Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
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Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
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Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
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Nicholson J, Browning S, Orloff S, McDougal J. Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry. J Immunol Methods. 1993; 160:215-218. (Biology).
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Nicholson J, Jones B, Hubbard M. CD4 Tlymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993; 14:598-605. (Biology).
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Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.
