Skip to main content Skip to navigation
Annexin V Binding Buffer, 10X concentrate

BD Pharmingen™ Annexin V Binding Buffer, 10X concentrate

Product Details
Down Arrow Up Arrow


The Annexin V Binding Buffer, 10X concentrate has been formulated for use in flow cytometric assays utilizing Annexin V.  The Annexin V Binding Buffer is a 10X concentrate composed of a 0.2 µm sterile filtered 0.1M Hepes (pH 7.4), 1.4M NaCl, and 25 mM CaCl2 solution.  Prior to staining cells, an appropriate quantity of a 1X working solution should be made by diluting the 10X concentrate 1:10 with distilled water.  Any remaining 1X solution at the end of the experiment should be discarded.

Product Notices

  1. Please refer to for technical protocols.
556454 Rev. 4
Citations & References
Down Arrow Up Arrow

Development References (1)

  1. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995; 184(1):39-51. (Methodology: Apoptosis, Flow cytometry). View Reference
556454 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.