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BD Transduction Laboratories™ Purified Mouse Anti-DHFR
Clone 49/DHFR (RUO)
Western blot analysis of DHFR on RSV-3T3 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of DHFR.
Western blot analysis of DHFR on RSV-3T3 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of DHFR.
Product Details
BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG1
Cow DHFR aa. 1-186
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Not Recommended)
21 kDa
250 µg/ml
AB_398020
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610697 Rev. 1
Antibody Details
49/DHFR
Dihydrofolate reductase (DHFR) regenerates tetrahydrofolate from dihydrofolate in the presence of NADPH. Tetrahydrofolate is the coenzyme for thymidilate synthetase in the biosynthesis of thymidine and is also critical for the synthesis of amino acids and purines. DHFR is a protein of 186 amino acids that is highly conserved among different organisms. DHFR levels change during the cell cycle, with the highest content during the G1/S transition. Expression of DHFR tightly correlates with the turnover rate of its mRNA. Because dividing cells require a continuous supply of thymidine, DHFR has been a target for anticancer drugs. The folic acid antagonists aminopterin and amethopterin (methotrexate), widely used in cancer treatments, inhibit DHFR. However, over long treatments, treated cells often amplify the DHFR gene, producing drug-resistant cells.
610697 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610697 Rev.1
Citations & References
View product citations for antibody "610697" on CiteAb
Development References (5)
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Eymin B, Gazzeri S, Brambilla C, Brambilla E. Distinct pattern of E2F1 expression in human lung tumours: E2F1 is upregulated in small cell lung carcinoma. Oncogene. 2001; 20(14):1678-1687. (Clone-specific: Western blot). View Reference
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Israel DI, Kaufman RJ. Dexamethasone negatively regulates the activity of a chimeric dihydrofolate reductase/glucocorticoid receptor protein. Proc Natl Acad Sci U S A. 1993; 90(9):4290-4294. (Biology). View Reference
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Masters JN, Attardi G. The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase. Gene. 1983; 21(1-2):59-63. (Biology). View Reference
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Tang MS, Pao A, Zhang XS. Repair of benzo(a)pyrene diol epoxide- and UV-induced DNA damage in dihydrofolate reductase and adenine phosphoribosyltransferase genes of CHO cells. J Biol Chem. 1994; 269(17):12749-12754. (Biology). View Reference
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de Wind N, Dekker M, Claij N. HNPCC-like cancer predisposition in mice through simultaneous loss of Msh3 and Msh6 mismatch-repair protein functions. Nat Genet. 1999; 23(3):359-362. (Clone-specific: Western blot). View Reference
610697 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.