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BD Transduction Laboratories™ Purified Mouse Anti-Mena
Clone 21/Mena (RUO)
Western blot analysis of Mena on a SW13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Mena antibody.
Immunofluorescence staining of human endothelial cells.
Immunohistochemical staining on rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment (40X magnification).
Western blot analysis of Mena on a SW13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Mena antibody.
Immunofluorescence staining of human endothelial cells.
Immunohistochemical staining on rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment (40X magnification).
Product Details
BD Transduction Laboratories™
Mammalian Ena (Enabled)
Human (QC Testing), Mouse, Rat, Chicken (Tested in Development)
Mouse IgA
Mouse Mena aa. 415-541
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
140, 88, 80 kDa
250 µg/ml
AB_398016
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
HRP Goat Anti-Mouse Ig RUO
Size
1 ML
Cat No.
554002
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
610693 Rev. 1
Antibody Details
21/Mena
Abl and Disabled (Dab) are essential for correct axonal connections during Drosophila development. Mutations in Enabled (Ena) rescue the genetic defects caused by mutated Abl and Dab. Mena (Mammalian Ena) is a protein with significant homology to Eva, VASP, and WASP proteins. The murine Mena gene predicts a protein of 541 amino acids. However, two additional exons that could introduce 246 amino acids onto the C-terminal region have been identified. Homology searches identified two EVH domains (Ena-Vasp homology), one near each terminus of Mena. Antibodies to Mena detect 80 kDa, 88 kDa, and 140 kDa protein with the 80 kDa and 88 kDa proteins being widely expressed and the 140 kDa protein reportedly mainly found in brain tissue. Like VASP, Mena is localized to focal contacts and, when ectopically expressed, induces the formation of F-actin outgrowths in fibroblasts.
This antibody is routinely tested by Western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
610693 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610693 Rev.1
Citations & References
View product citations for antibody "610693" on CiteAb
Development References (4)
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Drees B, Friederich E, Fradelizi J, Louvard D, Beckerle MC, Golsteyn RM. Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins. J Biol Chem. 2000; 275(29):22503-22511. (Biology: Immunofluorescence, Western blot). View Reference
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Garcia Arguinzonis MI, Galler AB, Walter U, Reinhard M, Simm A. Increased spreading, Rac/p21-activated kinase (PAK) activity, and compromised cell motility in cells deficient in vasodilator-stimulated phosphoprotein (VASP). J Biol Chem. 2002; 277(47):45604-45610. (Biology: Western blot). View Reference
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Gertler FB, Niebuhr K, Reinhard M, Wehland J, Soriano P. Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics. Cell. 1996; 87(2):227-239. (Biology). View Reference
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Howe AK, Hogan BP, Juliano RL. Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion. J Biol Chem. 2002; 277(41):38121-38126. (Biology: Western blot). View Reference
610693 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.