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BD Transduction Laboratories™ Purified Mouse Anti-IAK1
Clone 4/IAK1 (RUO)
Western blot analysis of IAK1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-IAK1 antibody.
Immunofluorescence staining of human endothelial cells.
Western blot analysis of IAK1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-IAK1 antibody.
Immunofluorescence staining of human endothelial cells.
Product Details
BD Transduction Laboratories™
Aurora-A Kinase
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Mouse IAK1 aa. 8-116
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
46 kDa
250 µg/ml
AB_398252
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Jurkat Cell Lysate RUO
Size
500 µg
Cat No.
611451
HRP Goat Anti-Mouse Ig RUO
Size
1 ML
Cat No.
554002
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
610939 Rev. 1
Antibody Details
4/IAK1
Cell division is a tightly regulated process that ensures the segregation of chromosomes into daughter cells. Essential to this regulation is the modification of cell cycle components by reversible phosphorylation. Ipl1 and aurora are two related kinases isolated from S.cerevisiae and Drosophila, respectively. Inactivation of these kinases results in abnormal chromosome segregation and disruption of the centrosome. A structurally and functionally similar kinase, IAK1 (Ipl1- and Aurora-related kinase 1), is a regulator of mammalian chromosome segregation. Although IAK1 may be present in the cytoplasm, it is detected on the centrosome following duplication and also associates with the spindle microtubules from metaphase through cell division. Expression of IAK1 is stringently regulated during the cell cycle. Both mRNA and protein are initially expressed in S-phase, are elevated during M-phase, and are undetectable following completion of mitosis. Increasing evidence suggests that IAK1 belongs to a novel subfamily of the ser/thr kinase superfamily. Although mutational analysis of IAK1 will directly determine its function, it appears to be a key player in the control of cell division.
610939 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610939 Rev.1
Citations & References
View product citations for antibody "610939" on CiteAb
Development References (4)
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Gopalan G, Chan CS, Donovan PJ. A novel mammalian, mitotic spindle-associated kinase is related to yeast and fly chromosome segregation regulators.. J Cell Biol. 1997; 138(3):643-56. (Biology). View Reference
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Katayama H, Ota T, Jisaki F. Mitotic kinase expression and colorectal cancer progression. J Natl Cancer Inst. 1999; 91(13):1160-1162. (Biology: Western blot). View Reference
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Kiat LS, Hui KM, Gopalan G. Aurora-A kinase interacting protein (AIP), a novel negative regulator of human Aurora-A kinase. J Biol Chem. 2002; 277(47):4558-45565. (Biology: Western blot). View Reference
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Sakai H, Urano T, Ookata K. MBD3 and HDAC1, two components of the NuRD complex, are localized at Aurora-A-positive centrosomes in M phase. J Biol Chem. 2002; 277(50):48714-48723. (Biology: Immunofluorescence). View Reference
610939 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.