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BrdU In-Situ Detection Kit II

BD Pharmingen™ BrdU In-Situ Detection Kit II

Product Details
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BD Pharmingen™
Flow cytometry, Immunohistochemistry, Intracellular staining (flow cytometry) (Tested During Development)

Preparation And Storage

All the components of the BrdU kit must be stored at 4°C. Some solutions require diluting or mixing prior to use.

Product Notices

  1. Please refer to for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to to access safety data sheets (SDS).
551321 Rev. 1
Citations & References
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Development References (21)

  1. Dolbeare F, Gratzner H, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci U S A. 1983; 80(18):5573-5577. (Biology). View Reference
  2. Dover R, and Patel K. Improved methodology for detecting bromodeoxyuridine in cultured cells and tissue sections by immunocytochemistry. Histochemistry. 1994; 102(5):383-387. (Biology).
  3. Ezaki T. Antigen retrieval on formaldehyde-fixed paraffin sections: its potential drawbacks and optimization for double immunostaining. Micron. 2000; 31(6):639-649. (Biology).
  4. Fukuta K, Iwasaka T, Hachisuga T, Sugimori H, Tsugitomi H, Mutoh F. Immunocytochemical detection of S-phase cells in normal and neoplastic cervical epithelium by anti-BrdU monoclonal antibody. Anal Quant Cytol Histol. 1990; 12(2):135-138. (Biology).
  5. Gratzner HG. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982; 218:474. (Biology).
  6. Holm M, Thomsen M, Hoyer M, Hokland P. Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells. Cytometry. 1998; 32(1):28-36. (Biology). View Reference
  7. Hume WJ. A reproducible technique combining tritiated thymidine autoradiography with immunodetection of bromodeoxyuridine for double labeling studies of cell proliferation in paraffin sections of tissues. Cell Tissue Kinet. 1990; 23(3):161-168. (Biology).
  8. Mckinley J N, Knott K K, and Thompson H J. Effect of fixation and epitope retrieval on BrdU indices in mammary carcinomas. J Histochem cytochem. 2000; 48(3):355-362. (Biology).
  9. Morstyn G, Pyke K, Gardner J, Ashcroft R, de Fazio A, and Bhathal p. Immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody. J Hictochem Cytochem. 1986; 34(6):697-701. (Biology).
  10. Qin Y, and Willems G. Comparison of the classical autoradiographic and the immunohistochemical methods with BrdU for measuring proliferation parameters in colon cancer. Anticancer Res. 1993; 13(3):731-735. (Biology).
  11. Raza A, Ucar K, Bhayana R, Kempski M, and Preisler HL. Utility and sensitivity of anti-BrdU antibodies in assessing S-phase cells compared to autoradiography. Cell Biochem funct. 1985; 3(2):149-153. (Biology).
  12. Robertson H, Wheeler J, and Morley AR. In vivo bromodeoxyuridine incorporation in normal mouse kidney: immunohistochemical detection and measurement of labeling indices. Histochem J. 1990; 22:209-214. (Biology).
  13. Rocha B, Penit C, Baron C, Vasseur F, Dautigny N, Freitas AA. Accumulation of bromodeoxyuridine-labeled cells in central and peripheral lymphoid organs: minimal estimates of production and turnover rates of mature lymphocytes. Eur J Immunol. 1990; 20(8):1697-1708. (Biology). View Reference
  14. Sasaki K, Ogino T, and Takahashi M. Immunological determination of labeling index on human tumor tissue sections using monoclonal anti-BrdU antibody. Stain Technol. 1986; 61(3):155-161. (Biology).
  15. Schutte B, Reynders M M, Bosman F T, and Blijham GH. Studies with antibromodeoxyuridine antibodies: II: Simultaneous immunocytchemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa. J Histochem cytochem. 1987; 35(3):371-374. (Biology).
  16. Thoman ML. Early steps in T cell development are affected by aging. Cell Immunol. 1997; 178(2):117-123. (Biology). View Reference
  17. Tischler AS. Triple immunohistochemical staining for bromodeoxyuridine and catecholamine biosynthetic enzymes using microwave antigen retrieval. J Histochem cytochem. 1995; 43(1):1-4. (Biology).
  18. Tough DF, Sprent J. Turnover of naive- and memory-phenotype T cells. J Exp Med. 1994; 179(4):1127-1135. (Biology). View Reference
  19. Yu CC, Woods AL, and Levison DA. The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications. Histochem J. 1992; 24(3):121-131. (Biology).
  20. deFazio A, Leary JA, Hedley DW, Tattersall MH. Immunohistochemical detection of proliferating cells in vivo. J Histochem Cytochem. 1987; 35(5):571-577. (Biology). View Reference
  21. von Boehmer H, Hafen K. The life span of naive alpha/beta T cells in secondary lymphoid organs. J Exp Med. 1993; 177(4):891-896. (Biology). View Reference
View All (21) View Less
551321 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.