Purified Mouse Anti-Human CD137

BD Pharmingen™ Purified Mouse Anti-Human CD137

Name: Purified Mouse Anti-Human CD137
Brand: BD Pharmingen™
SKU: 555955

Clone 4B4-1

(RUO)

Profile of Con A-stimulated peripheral blood mononuclear cells (PBMC) analyzed by flow cytometry. Second step staining with Cat. No. 555988.

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Product Details

Brand: BD Pharmingen™

Alternative Name: 4-1BB, ILA, TNFRSF9

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Recombinant Human 4-1BB Fusion Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

Workshop Number: VI C-7

RRID: AB_396251

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

Purified Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 555746

FITC Goat Anti-Mouse IgG/IgM RUO

Size 0.5 mg Cat No. 555988

555955 Rev. 6

Antibody Details

4B4-1

The 4B4-1 monoclonal antibody specifically binds to CD137 which is also known as 4-1BB, and ILA (induced by lymphocyte activation). CD137 is a type I transmembrane glycoprotein that belongs to the TNF/NGF receptor family. It is encoded by TNFRSF9 (tumor necrosis factor receptor superfamily, member 9). CD137 is expressed on activated T cells, B cells, monocytes, and follicular dendritic cells. CD137 plays roles in the costimulation, differentiation and survival of T cells and B cells.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555955 Rev. 6

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

555955 Rev.6

Citations & References

View product citations for antibody "555955" on CiteAb

Development References (5)

Garni-Wagner BA, Lee ZH, Kim YJ, Wilde C, Kang CY, Kwon BS. 4-1BB is expressed on CD45RAhiROhi transitional T cell in humans. Cell Immunol. 1996; 169(1):91-98. (Biology). View Reference
Hurtado JC, Kim SH, Pollok KE, Lee ZH, Kwon BS. Potential role of 4-1BB in T cell activation. Comparison with the costimulatory molecule CD28. J Immunol. 1995; 155(7):3360-3367. (Biology). View Reference
Hurtado JC, Kim YJ, Kwon BS. AAI Meeting, Abstract #841. San Francisco: 1997.
Kim YJ, Kim SH, Kwon BS. AAI Meeting, Abstract #838. San Francisco: 1997.
Zhou Z, Kim S, Hurtado J, et al. Characterization of human homologue of 4-1BB and its ligand. Immunol Lett. 1995; 45(1-2):67-73. (Biology). View Reference

View All (5)

555955 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.