Flow cytometric analysis of Vβ 13 TCR expression on mouse peripheral blood lymphocytes. C57BL/6 lymph node cells simultaneously stained with FITC Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 553204), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033). The two-color contour plot depicting Vβ 13 TCR versus CD4 and CD8a expression was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
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Flow cytometric analysis of Vβ 13 TCR expression on mouse peripheral blood lymphocytes. C57BL/6 lymph node cells simultaneously stained with FITC Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 553204), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033). The two-color contour plot depicting Vβ 13 TCR versus CD4 and CD8a expression was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
Product Details
BD Pharmingen™
TCR V beta 13; TCR Vβ 13; TCR Vβ13; Vβ 13 T-cell Receptor
Mouse (QC Testing)
Mouse C57BR IgG1, κ
Not reported
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394706
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Recommended Assay Procedures
For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
FITC Mouse IgG1, κ Isotype Control RUO
Size
0.25 mg
Cat No.
550616
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PE Rat Anti-Mouse CD4 RUO
Size
0.2 mg
Cat No.
553049
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PE Rat Anti-Mouse CD8a RUO
Size
0.2 mg
Cat No.
553033
PE Rat Anti-Mouse CD4 RUO
Size
0.1 mg
Cat No.
553048
553204 Rev. 10
Antibody Details
MR12-3
The MR12-3 monoclonal antibody specifically binds to the Vβ 13 T-cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V13 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype.
553204 Rev. 10
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
553204 Rev.10
Citations & References
View product citations for antibody "553204" on CiteAb
Development References (3)
-
Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). View Reference
-
Chen D, Lee F, Cebra JJ, Rubin DH. Predominant T-cell receptor Vbeta usage of intraepithelial lymphocytes during the immune response to enteric reovirus infection.. J Virol. 1997; 71(5):3431-6. (Biology). View Reference
-
Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
553204 Rev. 10
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.