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BV510 Mouse Anti-Rat CD2
Product Details
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BD OptiBuild™
LFA-2; OX34; Cd2
Rat (Tested in Development)
Mouse IgG2a, κ
Rat T blasts from mixed lymphocyte reactions
Flow cytometry (Qualified)
0.2 mg/ml
AB_2742693
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
745076 Rev. 1
Antibody Details
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OX-34

The OX-34 antibody reacts with CD2 (LFA-2), a member of the immunoglobulin superfamily. In the rat, CD2 is expressed on thymocytes, T lymphocytes in spleen and lymph node, dendritic epidermal T cells, splenic macrophages, and NK cells, but not on B cells, most intestinal intraepithelial lymphocytes, or peritoneal and liver macrophages. CD2 can associate with the T-cell receptor complex, and it may function in both intercellular adhesion and signal transduction. In the rat, CD48 and CD59 have been identified as ligands for CD2. OX-34 mAb binds to the extracellular portion of CD2, and it blocks the binding of CD2 to CD48. While OX-34 antibody does not activate T cells, it partially blocks activation by immobilized mAbs to CD3 (clone G4.18) and αβ T-cell receptor (clone R73), and it partially inhibits allogeneic mixed lymphocyte reactions. Moreover, in vivo administration of OX-34 antibody depletes peripheral T cells and prevents allograft rejection.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

745076 Rev. 1
Format Details
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BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV510
Violet 405 nm
327 nm, 405 nm
512 nm
745076 Rev.1
Citations & References
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View product citations for antibody "745076" on CiteAb

Development References (11)

  1. Beyers AD, Spruyt LL, Williams AF. Molecular associations between the T-lymphocyte antigen receptor complex and the surface antigens CD2, CD4, or CD8 and . Proc Natl Acad Sci U S A. 1992; 89(7):2945-2949. (Biology). View Reference
  2. Brown MH, Preston S, Barclay AN. A sensitive assay for detecting low-affinity interactions at the cell surface reveals no additional ligands for the adhesion pair rat CD2 and CD48. Eur J Immunol. 1995; 25(12):3222-3228. (Biology). View Reference
  3. Clark SJ, Law DA, Paterson DJ, Puklavec M, Williams AF. Activation of rat T lymphocytes by anti-CD2 monoclonal antibodies. J Exp Med. 1988; 167(6):1861-1872. (Biology). View Reference
  4. Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Biology). View Reference
  5. Fangmann J, Schwinzer R, Wonigeit K. Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: predominance of T cell receptor alpha/beta+/CD2- cells and high expression of the RT6 alloantigen. Eur J Immunol. 1991; 21(3):753-760. (Biology). View Reference
  6. Hirahara H, Tsuchida M, Watanabe T, et al. Long-term survival of cardiac allografts in rats treated before and after surgery with monoclonal antibody to CD2. Transplantation. 1995; 59(1):85-90. (Biology). View Reference
  7. Jefferies WA, Green JR, Williams AF. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med. 1985; 162(1):117-127. (Immunogen: Flow cytometry, Functional assay, Immunoaffinity chromatography, Immunoprecipitation, Inhibition). View Reference
  8. Liversidge J, Dawson R, Hoey S, McKay D, Grabowski P, Forrester JV. CD59 and CD48 expressed by rat retinal pigment epithelial cells are major ligands for the CD2-mediated alternative pathway of T cell activation. J Immunol. 1996; 156(10):3696-3703. (Biology). View Reference
  9. Williams AF, Barclay AN, Clark SJ, Paterson DJ, Willis AC. Similarities in sequences and cellular expression between rat CD2 and CD4 antigens. J Exp Med. 1987; 165(2):368-380. (Biology). View Reference
  10. van den Brink MR, Hunt LE, Hiserodt JC. In vivo treatment with monoclonal antibody 3.2.3 selectively eliminates natural killer cells in rats. J Exp Med. 1990; 171(1):197-210. (Biology). View Reference
  11. van der Merwe PA, Brown MH, Davis SJ, Barclay AN. Affinity and kinetic analysis of the interaction of the cell adhesion molecules rat CD2 and CD48. EMBO J. 1993; 12(3):4945-4954. (Biology). View Reference
View All (11) View Less
745076 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.