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BD Horizon™ BV480 Mouse Anti-Human TCR γδ
Clone B1 (RUO)
Two-color flow cytometric analysis of TCR γδ expression on human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811) and either BD Horizon™ BV480 Mouse IgG1, κ Isotype Control (Cat. No. 565652; Left Plot) or BD Horizon BV480 Mouse Anti-Human TCR γδ antibody (Cat No. 566076/566084). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-color flow cytometric contour plot showing the correlated expression of TCR γδ (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of TCR γδ expression on human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811) and either BD Horizon™ BV480 Mouse IgG1, κ Isotype Control (Cat. No. 565652; Left Plot) or BD Horizon BV480 Mouse Anti-Human TCR γδ antibody (Cat No. 566076/566084). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-color flow cytometric contour plot showing the correlated expression of TCR γδ (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of TCR γδ expression on human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811) and either BD Horizon™ BV480 Mouse IgG1, κ Isotype Control (Cat. No. 565652; Left Plot) or BD Horizon BV480 Mouse Anti-Human TCR γδ antibody (Cat No. 566076/566084). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-color flow cytometric contour plot showing the correlated expression of TCR γδ (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of TCR γδ expression on human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811) and either BD Horizon™ BV480 Mouse IgG1, κ Isotype Control (Cat. No. 565652; Left Plot) or BD Horizon BV480 Mouse Anti-Human TCR γδ antibody (Cat No. 566076/566084). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-color flow cytometric contour plot showing the correlated expression of TCR γδ (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Horizon™
TCRgd; γδ TCR; TRG@, TRD@; TCRG, TCRD; TCR gamma delta
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739491
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with BD Horizon BV480 under optimum conditions, and unconjugated antibody and free BD Horizon BV480 were removed. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Companion Products
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
BV480 Mouse IgG1, k Isotype Control RUO
Size
50 µg
Cat No.
565652
566084 Rev. 1
Antibody Details
B1
The B1 monoclonal antibody specifically binds to the γδ T cell receptor (γδ TCR). This receptor complex consists of two disulfide-linked transmembrane glycoproteins, a γ chain (45-60 kDa) and a δ subunit (40-60 kDa). The γδ TCR is associated with the signal-transducing CD3 complex. The γδ TCR is expressed by thymocytes and by peripheral T cell subsets (γδ T cells) that are located in the blood, liver, skin and various lymphoid and mucosal tissues. γδ T cells contribute to both innate and adaptive immune responses to infections and tumors. Reports suggest that γδ T cells may also play roles in antigen presentation and the regulation of autoimmune responses.
The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.
566084 Rev. 1
Format Details
BV480
The BD Horizon Brilliant Violet™ 480 (BV480) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology fluorochrome has an excitation maximum (Ex Max) of 440-nm and an emission maximum (Em Max) of 479-nm. Driven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). The increased fluorescence intensity of BV480 and narrower emission spectra, make it a good alternative for BV510 or V500. Due to its excitation profile, BV480 also has less cross-laser excitation with the UV laser, resulting in less spillover into UV channels compared to BV510. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV480
440 nm
479 nm
566084 Rev.1
Citations & References
View product citations for antibody "566084" on CiteAb
Development References (6)
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Bai L, Picard D, Anderson B, et al. The majority of CD1d-sulfatide-specific T cells in human blood use a semiinvariant Vδ1 TCR.. Eur J Immunol. 2012; 42(9):2505-10. (Clone-specific: Flow cytometry). View Reference
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Bonneville M, O'Brien RL, Born WK. Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat Rev Immunol. 2110; 10(7):467-478. (Biology). View Reference
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Davodeau F, Peyrat MA, Houde I, et al. Surface expression of two distinct functional antigen receptors on human gamma delta T cells. Science. 1993; 260(5115):1800-1802. (Clone-specific). View Reference
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De Libero G. Sentinel function of broadly reactive human gamma delta T cells. Immunol Today. 1997; 18(1):22-26. (Biology). View Reference
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Del Porto P, D'Amato M, Fiorillo MT, Tuosto L, Piccolella E, Sorrentino R. Identification of a novel HLA-B27 subtype by restriction analysis of a cytotoxic gamma delta T cell clone.. J Immunol. 1994; 153(7):3093-100. (Clone-specific: Flow cytometry). View Reference
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Kabelitz D. γδ T-cells: cross-talk between innate and adaptive immunity. Cell Mol Life Sci. 2011; 68(14):2331-2333. (Biology). View Reference
566084 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.