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Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse CD319 antibody (Cat. No. 747997), APC CD19 on live mouse splenocytes (right figure), with Isotype Control (left figure). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
BD OptiBuild™ BV421 Rat Anti-Mouse CD319 (CRACC)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Companion Products
The 4G2 monoclonal antibody specifically recognizes CD319 which is also known as CD2-like receptor activating cytotoxic cells (CRACC), Signaling lymphocytic activation molecule 7 (Slamf7), or novel Ly9. CD319 (CRACC) is a single-pass type I transmembrane glycoprotein that is encoded by Slamf7 (SLAM family member 7) which belongs to the SLAM family within the CD2-subset of the immunoglobulin superfamily (IgSF) of cell surface receptors. This receptor is comprised of an extracellular region with one N-terminal Ig-like V-type (IgV) domain followed by one Ig-like C2-type (IgC2) domain. Its cytoplasmic tail contains two immunoreceptor tyrosine-based switch motifs (ITSMs). This receptor is variably expressed on activated CD4+ T cells and CD8+ T cells, NKT cells, NK cells, B cells, monocytes, macrophages, and dendritic cells. CD319 (CRACC) serves as a homotypic adhesion molecule that can self-associate and trigger cellular activation, such as cell-mediated cytotoxicity, when its cytoplasmic domains are associated with adaptor molecules including EWS-activated transcript 2 (EAT-2) and possibly EAT-2-related transducer (ERT). In cells that lack EAT-2, CD319 (CRACC) may exhibit an inhibitory function.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Development References (2)
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Cruz-Munoz ME, Dong Z, Shi X, Zhang S, Veillette A. Influence of CRACC, a SLAM family receptor coupled to the adaptor EAT-2, on natural killer cell function.. Nat Immunol. 2009; 10(3):297-305. (Immunogen). View Reference
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Guo H, Cruz-Munoz ME, Wu N, Robbins M, Veillette A. Immune cell inhibition by SLAMF7 is mediated by a mechanism requiring src kinases, CD45, and SHIP-1 that is defective in multiple myeloma cells.. Mol Cell Biol. 2015; 35(1):41-51. (Clone-specific). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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