BD OptiBuild™ BUV737 Mouse Anti-Human CD62L

The  DREG-56 monoclonal antibody specifically binds to CD62L. CD62L is a 76-95 kDa glycoprotein that is also referred to as L-selectin or LECAM-1. CD62L is expressed on neutrophils, monocytes, T- and B-lymphocyte subsets and NK cells. The DREG-56 antibody recognizes the same antigen as LAM-1, and specifically inhibits >90% of binding of human lymphocytes to high endothelial venules (HEV) in frozen sections of peripheral, but not mucosal lymphoid tissue. It thus defines L-selectin as a human lymphocyte homing receptor for peripheral lymph node HEV.

The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter.  Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).

Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.