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Biotin Rat Anti-Mouse IgM
Product Details
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BD Pharmingen™
Ighm; Igh-M; Immunoglobulin M; Igh6; muH; immunoglobulin heavy constant mu
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Pooled Mouse Ig
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

For the sandwich mouse IgM ELISA, biotin-conjugated R6-60.2 mAb is optimal for detection with purified anti-mouse IgM II/41 mAb (Cat. No. 553435) for capture. Biotinylated R6-60.2 mAb (Cat. No. 553406) may be used as a primary or secondary reagent in immunofluorescent staining. Biotinylated R6-60.2 mAb may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgM, we recommend FITC-conjugated mAb II/41 (Cat. No. 553437).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Please refer to for technical protocols.
553406 Rev. 13
Antibody Details
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The R6-60.2 antibody monoclonal antibody specifically binds to mouse Immunoglobulin M (IgM) of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. The R6-60.2 antibody has not been shown to stimulate B-cell proliferation.

553406 Rev. 13
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553406 Rev.13
Citations & References
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Development References (3)

  1. BD Biosciences Pharmingen. Unpublished results. .
  2. Gavin AL, Duong B, Skog P, et al. δBAFF, a splice isoform of BAFF, opposes full-length BAFF activity in vivo in transgenic mouse models. J Immunol. 2005; 75(1):319-328. (Clone-specific). View Reference
  3. Touma M, Keskin DB, Shiroki F, et al. Impaired B cell development and function in the absence of IκBNS. J Immunol. 2011; 187(8):3942-3952. (Clone-specific). View Reference
553406 Rev. 13

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.