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      Biotin Rat Anti-Mouse IgG2a
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
      Pooled BALB/c and C57BL/6 mouse Ig
      ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
      250 µg/ml
      380793
      AB_393612
      Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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      Recommended Assay Procedures

      This antibody conjugate has been tested as a detecting reagent in ELISA (~2 µg/ml) to assure purity and reactivity.

      For immunohistochemical staining on acetone-fixed frozen sections, a dilution range of 1:25 - 1:100 is suggested.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550332 Rev. 5
      Antibody Details
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      R19-15

      The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] allotype and weaker reactivity to Igh-I[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

      550332 Rev. 5
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      550332 Rev.5
      Citations & References
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      View product citations for antibody "550332" on CiteAb

      Development References (2)

      1. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology). View Reference
      2. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology). View Reference
      550332 Rev. 5

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.