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Multicolor flow cytometric analysis of CD115 (CSF-1R) expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with BD Horizon™ BV421 Rat Anti-Mouse CD11b antibody (Cat. No. 562605) and either Biotin Rat IgG1, κ Isotype Control (Cat. No. 553923; Left Plot) or Biotin Rat Anti-Mouse CD115 (CSF-1R) antibody (Cat. No. 567598; Right Plot) at 0.25 µg/test, followed by PE Streptavidin (Cat. No. 554061). A bivariate pseudocolor density plot showing the correlated expression of CD115 (CSF-1R) [or Ig Isotype control staining] versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ Biotin Rat Anti-Mouse CD115 (CSF-1R)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
The T38-320 monoclonal antibody specifically binds to CD115 which is also known as Colony stimulating factor 1 Receptor (CSF-1R) or Macrophage colony-stimulating factor 1 receptor (M-CSFR). This type I transmembrane glycoprotein is a receptor tyrosine kinase (RTK) that belongs to the Ig superfamily. It is expressed on a variety of cells including those committed to the mononuclear phagocyte lineage, such as, monocytes, macrophages, and osteoclasts. CSF-1 binds to and signals through CSF-1R homodimers which undergo tyrosine autophosphorylation and transduce downstream signaling pathways resulting in cytoskeletal reorganization and gene expression. CSF-1R activation stimulates the proliferation, differentiation, and survival of cells within the mononuclear phagocyte system. Interleukin-34 (IL-34) is another ligand for CD115 that can induce similar, as well as, some different biological responses by CD115-positive target cells.
Development References (7)
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Breslin WL, Strohacker K, Carpenter KC, Haviland DL, McFarlin BK. Mouse blood monocytes: standardizing their identification and analysis using CD115. J Immunol Methods. 2013; 390(1-2):1-8. (Biology). View Reference
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De Schepper S, Verheijden S, Aguilera-Lizarraga J, et al. Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis.. Cell. 2018; 175(2):400-415.e13. (Clone-specific: Flow cytometry). View Reference
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Fend L, Accart N, Kintz J et al. Therapeutic effects of anti-CD115 monoclonal antibody in mouse cancer models through dual inhibition of tumor-associated macrophages and osteoclasts. PLoS ONE. 2013; 8(9):e73310. (Biology). View Reference
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Huang B, Pan PY, Li Q et al. Gr-1+CD115+ immature myeloid suppressor cells mediate the development of tumor-induced T regulatory cells and T-cell anergy in tumor-bearing host. Cancer Res. 2006; 15(66):1123-1131. (Biology). View Reference
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Muench DE, Olsson A, Ferchen K, et al. Mouse models of neutropenia reveal progenitor-stage-specific defects. Nature. 2020; 582(7810):109-114. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
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Rothwell VM, Rohrschneider LR. Murine c-fms cDNA: cloning, sequence analysis and retroviral expression. Oncogene Res. 1987; 1(4):311-324. (Biology). View Reference
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Zhang Z, Dong L, Jia A, et al. Glucocorticoids Promote the Onset of Acute Experimental Colitis and Cancer by Upregulating mTOR Signaling in Intestinal Epithelial Cells.. Cancers (Basel). 2020; 12(4):E945. (Clone-specific: Flow cytometry). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.