Biotin Mouse Anti-Rat Ig, κ Light Chain

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BD Pharmingen™ Biotin Mouse Anti-Rat Ig, κ Light Chain

Name: Biotin Mouse Anti-Rat Ig, κ Light Chain
Brand: BD Pharmingen™
SKU: 553871

Clone MRK-1

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Rat (QC Testing)

Isotype: Mouse IgG1

Immunogen: Pooled rat Ig

Application: ELISA Detection (Routinely Tested), Flow cytometry, Immunohistochemistry-frozen (Tested During Development)

Concentration: 0.5 mg/ml

RRID: AB_395104

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

ELISA: For detection of rat IgG1, G2a, G2b, G2c, IgA, or IgM in a sandwich ELISA, biotinylated mAb MRK-1 (can be cocktailed with biotin-conjugated anti-rat Ig λ light chain, clone G33-11) is optimal for detection with purified anti-rat Ig isotype-specific mAbs (RG11/39.4, Cat. No. 553889; RG7/1.30, Cat. No. 553893; RG7/11.1, Cat. No. 553897 or G15-337, Cat. No. 553882; A92-1, [available by custom order]; A93-2, [available by custom order]; G53-238, Cat. No. 553885) for capture, respectively.

Flow Cytometry: Biotin-conjugated MRK-1 mAb may be used as a primary or secondary reagent in immunofluorescent staining.

Immunohistochemistry: For use as a second-step in IHC, we suggest RG7/7.6 mAb (anti-rat and -hamster Ig κ light chain) in our special formulation for immunohistochemistry, Cat. No. 550336.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

553871 Rev. 13

Antibody Details

MRK-1

The MRK-1 monoclonal antibody reacts specifically with rat Igs bearing κ light chains. It does not react with rat immunoglobulin λ light chains or heavy chains. The MRK-1 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.

553871 Rev. 13

Format Details

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

Format: Biotin

553871 Rev.13

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.