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Biotin Mouse Anti-Mouse IgG2a [b]
Product Details
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BD Pharmingen™
Igh-1 [b]; Igh-1; Igh-1a; Ighg2a; Immunoglobulin heavy constant gamma 2A
Mouse (QC Testing)
Mouse BALB/c IgG3, κ
Not Reported
ELISA Detection (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553504 Rev. 11
Antibody Details
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This antibody reacts specifically with mouse IgG2a of Igh-C [b] haplotype (e.g., C57BL/6, C57BL/10, NOD, SJL). It has been reported not to react with IgG2a of the a, c, d, e, f, g, h, j, n, o, or p haplotypes (e.g., A, AKR, BALB/c, C3H/He, C58, CBA, DBA/1, DBA/2, NZB, SWR). Molecular genetic analyses suggest that the Igh-1 [b] allele, which encodes IgG2a [b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

553504 Rev. 11
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553504 Rev.11
Citations & References
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Development References (4)

  1. Huang CM, Parsons M, Oi VT, Huang HJ, Herzenberg LA. Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):311-321. (Biology). View Reference
  2. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology). View Reference
  3. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology). View Reference
  4. Parsons M, Oi VT, Huang CM, Herzenberg LA. Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):323-334. (Biology). View Reference
View All (4) View Less
553504 Rev. 11

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.