-
Your selected country is
Sweden
- Change country/language
Old Browser
This page has been recently translated and is available in French now.
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Multiparameter flow cytometric analysis of CD75/CD75s expression on human peripheral blood leucocyte populations. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer (Cat. No. 559759) to lyse erythrocytes and washed. The leucocytes were then stained with either Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD75/CD75s antibody (Cat. No. 566350; Right Plot). Flow cytometric contour plots showing the correlated expression of CD75/CD75s (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Two-color flow cytometric analysis of CD75/CD75s expression on human peripheral blood lymphocytes. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer (Cat. No. 559759) to lyse erythrocytes and washed. The leucocytes were then stained with BD Horizon™ BV421 Mouse Anti-Human CD19 antibody (Cat. No. 562440/562441) and either Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD75/CD75s antibody (Cat. No. 566350; Right Plot).The flow cytometric contour plots showing the correlated expression of CD75/CD75s (or Ig Isotype control staining) versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CD75/CD75s
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CD75/CD75s
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The ZB55 monoclonal antibody specifically recognizes the carbohydrate CD75 (lactosamines) and CD75s (sialylated lactosamines) antigens which are expressed in association with various glycolipids and glycoproteins. CD75 is expressed on mature B cells and germinal center B lymphocytes whereas CD75s is expressed on mature B cells. CD75 expression is lost on B cells differentiating to plasma cells whereas CD75s expression is lost on both germinal center B cells and B cells differentiating into plasma cells. CD75 and CD75s may be expressed by some T cells and can be useful markers for distinguishing different types of malignant B cells.
Development References (7)
-
CDw75 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:169-171.
-
Distler U, Souady J, Hülsewig M, et al. Tumor-associated CD75s- and iso-CD75s-gangliosides are potential targets for adjuvant therapy in pancreatic cancer.. Mol Cancer Ther. 2008; 7(8):2464-75. (Biology). View Reference
-
Jones M, Mason DY. B-cell Antigens: Section report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:127-130.
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
-
Schwartz-Albiez R. Carbohydrate and lectin: Section report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:149-164.
-
Stamenkovic I, Sgroi D, Aruffo A, Sy MS, Anderson T. The B lymphocyte adhesion molecule CD22 interacts with leukocyte common antigen CD45RO on T cells and alpha 2-6 sialyltransferase, CD75, on B cells. Cell. 1991; 66(6):1133-1144. (Biology). View Reference
-
Zola H, Swart B, Nicholson I, Voss E. CD75, CD75s, CDw76. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:168.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.