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Flow cytometric analysis of CD6 expression on human peripheral blood lymphocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD6 (Cat. No. 564260; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed on a BD™ LSR II.


BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CD6

Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The M-T605 monoclonal antibody specifically binds to CD6. CD6 is a 100-130 kDa type I transmembrane glycoprotein present on more than 80% of mature T cells and a subset of B cells. CD6 is also weakly expressed on cortical thymocytes. CD6 binds to CD166 and reportedly plays roles in cellular adhesion, costimulation of T lymphocyte proliferation, and the regulation of lymphocyte apoptosis. The antibody is useful for investigation of CD6+ B-cell function.
Development References (6)
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Bott CM, Doshi JB, Li LL, McMurtry SA, Sanders JL, Fox DA. Transcriptional regulation of CD6 expression on human T lymphocytes by phorbol ester. J Immunol. 1994; 153(1):1-9. (Biology). View Reference
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Endres N, Riethmuller G, Rieber EP. Functional characterization of a novel B-cell subsetdefined by the CD6 antigen. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:340.
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Rasmussen RA, Counts SL, Daley JF, Schlossman SF. Isolation and characterization of CD6- T cells from peripheral blood. J Immunol. 1994; 152(2):527-536. (Biology). View Reference
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Reiter C. Cluster report: CD6. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:339.
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Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.