Flow cytometric analysis of CD51 expression on human melanoma M21 cells. Cells from the human melanoma M21 cell line were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD51 antibody (Cat. No. 565835; solid line histogram). The fluorescence histogram showing CD51 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable M21 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD51 expression on human melanoma M21 cells. Cells from the human melanoma M21 cell line were stained with either Alexa Fluor™ 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human CD51 antibody (Cat. No. 565835; solid line histogram). The fluorescence histogram showing CD51 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable M21 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD51 expression on human fibrosarcoma HT-1080 cells. Cells from the human HT-1080 (Fibrosarcomma, ATCC CCL-121) cell line were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD51 antibody (solid line histogram). The fluorescence histogram showing CD51 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable HT-1080 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD51 expression on human fibrosarcoma HT-1080 cells. Cells from the human HT-1080 (Fibrosarcomma, ATCC CCL-121) cell line were stained with either Alexa Fluor™ 647 Mouse IgG2a, κ Isotype Control (dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human CD51 antibody (solid line histogram). The fluorescence histogram showing CD51 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable HT-1080 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD51 expression on human melanoma M21 cells. Cells from the human melanoma M21 cell line were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD51 antibody (Cat. No. 565835; solid line histogram). The fluorescence histogram showing CD51 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable M21 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD51 expression on human melanoma M21 cells. Cells from the human melanoma M21 cell line were stained with either Alexa Fluor™ 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human CD51 antibody (Cat. No. 565835; solid line histogram). The fluorescence histogram showing CD51 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable M21 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD51 expression on human fibrosarcoma HT-1080 cells. Cells from the human HT-1080 (Fibrosarcomma, ATCC CCL-121) cell line were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD51 antibody (solid line histogram). The fluorescence histogram showing CD51 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable HT-1080 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD51 expression on human fibrosarcoma HT-1080 cells. Cells from the human HT-1080 (Fibrosarcomma, ATCC CCL-121) cell line were stained with either Alexa Fluor™ 647 Mouse IgG2a, κ Isotype Control (dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human CD51 antibody (solid line histogram). The fluorescence histogram showing CD51 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable HT-1080 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
Integrin alpha-V; alphaV; αv; ITGAV; MSK8; VNRA; VTNR
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human Melanoma Cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
P103
AB_2739375
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
100 Tests
Cat No.
557715
565835 Rev. 2
Antibody Details
NKI-M9
The NKI-M9 monoclonal antibody specifically binds to CD51, which is also known as Integrin alpha-V (ITGAV, αv), or Vitronectin receptor subunit alpha (VNRA, VTNR). CD51 is a type I transmembrane glycoprotein that is cleaved into 125 kDa and 25 kDa subunits which are disulfide linked. CD51 can form heterodimers with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 is expressed on platelets, osteoclasts, endothelial cells, fibroblasts, macrophages, and some B cells, as well as neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs can serve as ligands for CD51. In association with various integrin β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. The CD51 adhesion molecule plays important roles in leukocyte activation and localization, bone absorption, and angiogenesis.
565835 Rev. 2
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
565835 Rev.2
Citations & References
View product citations for antibody "565835" on CiteAb
Development References (5)
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Bodary SC, McLean JW. The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J Biol Chem. 1990; 265(11):5938-5941. (Biology). View Reference
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Parravicini CL, Soligo D, Berti E, Cattoretti G, Gaiera G, Vago L. Immunohistochemical reactivity of anti-platelet mAb in normal human tissues and bone marrow. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:981-985.
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Suzuki S, Argraves WS, Arai H. Amino acid sequence of the vitronectin receptor alpha subunit and comparative expression of adhesion receptor mRNAs. J Biol Chem. 1987; 262(29):14080-14085. (Biology). View Reference
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Suzuki S, Argraves WS, Pytela R. cDNA and amino acid sequences of the cell adhesion protein receptor recognizing vitronectin reveal a transmembrane domain and homologies with other adhesion protein receptors. Proc Natl Acad Sci U S A. 1986; 83(22):8614-8618. (Biology). View Reference
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von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.
565835 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.