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FITC BrdU Flow Kit

BD Pharmingen™ FITC BrdU Flow Kit

(RUO)
Product Details
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BD Pharmingen™
Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
RUO
AB_2617060


Preparation And Storage

Antibody, buffers, and 7-AAD. Store the fluorochrome-conjugated anti-BrdU antibody, BD Cytofix/Cytoperm Buffer, BD Perm/Wash Buffer, BD Cytoperm Permeabilization Buffer Plus, and 7-AAD at 2 to 8°C. Keep the fluorochrome-conjugated anti-BrdU antibody and 7-AAD protected from light. Unused portions of 1X BD Cytofix/Cytoperm Buffer can be stored at 2 to 8°C. BrdU and DNase. Store the BrdU and DNase at –80°C. The BrdU solution has been shown to be stable for up to 4 months at 2 to 8°C, or it can be refrozen. Avoid multiple freeze-thaw cycles. DNase stock solution (1 mg/mL) may be refrozen once before it loses activity

559619 Rev. 1
Citations & References
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Development References (27)

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  8. Holm M, Thomsen M, Hoyer M, Hokland P. Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells. Cytometry. 1998; 32(1):28-36. (Biology). View Reference
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  11. Lakhanpal S, Gonchoroff NJ, Katzmann JA, Handwerger BS. A flow cytofluorometric double staining technique for simultaneous determination of human mononuclear cell surface phenotype and cell cycle phase. J Immunol Meth. 1987; 96:35-40. (Biology).
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559619 Rev. 1

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