BD Pharmingen™ Dog Activated T Lymphocyte Cocktail
Description
The Dog Lymphocyte Activation Cocktail is a three-color reagent cocktail designed to identify dog activated lymphocytes by direct immunofluorescent staining with flow cytometric analysis. The LSM 8.358 antibody reacts with a CD3-like T-cell receptor-associated cell-surface antigen found on thymocytes and peripheral T lymphocytes. The LSM 11.425 antibody was generated against cells derived from canine peripheral lymph nodes and has been used as a prognostic tool in dog B cell lymphoma studies. The CTL 2.58 antibody was generated by using whole cell immunizations of IL-2 dependent feline T cell lines stimulated with PHA and Con A. This clone reacts with dog and is referred to as the T Cell Activation Marker.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| APC Mouse anti-Dog Pan T Cell | N/A | 51-590-9005060 | N/A |
| Multiple Mouse anti-Dog B Cells | N/A | 51-550-9003756 | N/A |
| FITC Mouse anti-Dog Activated T cells | N/A | 51-540-9005068 | N/A |
Development References (3)
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Cobbold S, Metcalfe S. Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994; 43:137-154. (Clone-specific). View Reference
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Gebhard GH, Carter PB. Identification of canine T-lymphocyte subsets with monoclonal antibodies. Vet Immunol Immunopathol. 1992; 33:187-199. (Biology). View Reference
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Ruslander DA, Gebhard DH, Tompkins MB, Grindem CB, Page RL. Immunophenotypic characterization of canine lymphoproliferative disorders. In Vivo. 1997; 11(2):169-172. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.