Product Details
Description
Naturally occurring human CD4+ regulatory T cells (Tregs) are critical mediators of self-tolerance and suppress aberrant or inappropriate immune responses that can lead to conditions such as type 1 diabetes, rheumatoid arthritis, and graft-vs-host disease. The BD FastImmune™ kit measures expression of the activation markers CD154 and/or CD69 on effector T cells. Reduced expression of these markers in the presence of Tregs can indicate Treg suppressive capacity. The assay is performed in 96-well microplates using peripheral blood mononuclear cells (PBMCs) activated in the presence and absence of Tregs. It is a short-term assay that can be set up and analyzed in approximately one day. The T-cell markers, CD3 and CD4, are included in the kit for T-cell gating purposes, and CD25 is included for defining the Treg population.
Contents: Regulatory T-Cell function cocktail (CD3 PerCP-Cy™5.5, CD4 FITC, CD25 PE)| CD69 PE-Cy™7| CD154 APC| EDTA.
Preparation And Storage
Store reagents at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.