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      Anti-Human CD4 FITC/CD38 PE/CD3 PerCP/HLA-DR APC with BD Trucount™ Tubes

      BD Multitest™ Anti-Human CD4 FITC/CD38 PE/CD3 PerCP/HLA-DR APC with BD Trucount™ Tubes

      (IVD)
      Product Details
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      BD Multitest™
      Human
      Flow cytometry
      IVD
      PBS containing bovine serum albumin, and 0.1% sodium azide.


      Description

      The CD4 antibody, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. The CD38 antibody, clone HB7, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the BJAB cell line. The CD3 antibody, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. The Anti–HLA-DR antibody, clone L243, is derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastoid B-cell line RPMI 8866.

      CD4 recognizes an antigen that interacts with class II molecules of the major histocompatibility complex (MHC) and is the primary receptor for the human immunodeficiency virus. The cytoplasmic portion of the antigen is associated with the protein tyrosine kinase p56lck.The CD4 antigen can regulate the function of the CD3 antigen/Tcell antigen receptor (TCR) complex. CD4 also reacts with monocytes/macrophages that have an antigen density lower than that on helper/inducer T lymphocytes. CD38 recognizes an integral membrane glycoprotein, Mr 45 kDalton (kDa), with a protein core of 35 kDa. CD3 recognizes the epsilon chain of the CD3 antigen/TCR complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa). Anti–HLA-DR recognizes a human class II MHC antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kDa, respectively. Anti–HLA-DR reacts with a nonpolymorphic HLA-DR epitope and does not cross-react with HLA-DQ or HLA-DP molecules.

      Preparation And Storage

      The MultiTEST reagent is supplied as a combination of CD4 FITC, CD38 PE, CD3 PerCP, and Anti–HLA-DR APC in 1.0 mL of PBS containing bovine serum albumin, and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      337087 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      CD4 FITC SK3 IgG1, κ 920
      HLA-DR APC 104D2 IgG1, 3815
      CD38 PE HB7 IgG1, κ N/A
      CD3 PerCP SK7 IgG1, κ N/A
      337087 Rev. 1
      Citations & References
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      337087 Rev. 1

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