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Purified Mouse Anti-c-Jun (pS63)
Purified Mouse Anti-c-Jun (pS63)

Western blot analysis of c-Jun (pS63) in human vascular endothelium.  Lysates from control (left panel) and calyculin A-treated (right panel) EA-hy 926 cells (Edgell, McDonald, Graham, 1983) were probed with purified Mouse Anti-c-Jun (pS63) monoclonal antibody at concentrations of 0.063, 0.032, and 0.016 μg/ml  (lanes 1, 2, and 3, respectively).  c-Jun (pS63) is identified as a band of 39 kDa in the treated cells.

Western blot analysis of c-Jun (pS63) in human vascular endothelium.  Lysates from control (left panel) and calyculin A-treated (right panel) EA-hy 926 cells (Edgell, McDonald, Graham, 1983) were probed with purified Mouse Anti-c-Jun (pS63) monoclonal antibody at concentrations of 0.063, 0.032, and 0.016 μg/ml  (lanes 1, 2, and 3, respectively).  c-Jun (pS63) is identified as a band of 39 kDa in the treated cells.

Product Details
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BD Pharmingen™
Human (QC Testing), Mouse, Rat (Predicted)
Mouse IgG1
Phosphorylated Human c-Jun
Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
39 kDa
0.25 mg/ml
AB_2249448
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
558036 Rev. 2
Antibody Details
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2/c-Jun/(S63)

The activator protein transcription factor (AP-1) was identified as a protein that recognizes specific sequences in the cis-control regions of the SV40 virus and the human metallothionein IIA gene.  AP-1 is composed of protein products of two different gene families: jun and fos.  The AP-1 transcription factor is either a homodimer of Jun proteins or a heterodimer of Jun and Fos proteins.  The transcriptional activity of Jun is enhanced by phosphorylation of serines 63 (S63) and 73 in its activation domain.  Phosphorylation at both sites is necessary for stimulating the activating function of Jun.  Jun is phosphorylated by JNK protein kinases that are activated by the same signals that potentiate Jun activity.

The 2/c-Jun/(S63) monoclonal antibody recognizes the phosphorylated S63 of c-Jun.

558036 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558036 Rev.2
Citations & References
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Development References (7)

  1. Behrens A, Sibilia M, Wagner EF. Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation. Nat Genet. 1999; 21:326-329. (Biology).
  2. Dunn C, Wiltshire C, MacLaren A, Gillespie DA. Molecular mechanism and biological functions of c-Jun N-terminal kinase signalling via the c-Jun transcription factor. Cell Signal. 2002; 14(7):585-593. (Biology). View Reference
  3. Lamph WW, Wamsley P, Sassone-Corsi P, Verma IM. Induction of proto-oncogene JUN/AP-1 by serum and TPA. Nature. 1988; 334(6183):629-631. (Biology). View Reference
  4. Shaulian E, Karin M. AP-1 in cell proliferation and survival. Oncogene. 2001; 20(19):2390-2400. (Biology).
  5. Smeal T, Binetruy B, Mercola D, et al. Oncoprotein-mediated signalling cascade stimulates c-Jun activity by phosphorylation of serines 63 and 73. Mol Cell Biol. 1992; 12(8):3507-3513. (Biology).
  6. Weiss C, Bohmann D. Deregulated repression of c-Jun provides a potential link to its role in tumorigenesis. Cell Cycle. 2004; 3(2):111-113. (Biology).
  7. Wisdom R, Johnson RS, Moore C. c-Jun regulates cell cycle progression and apoptosis by distinct mechanisms. EMBO J. 1999; 18(1):188-197. (Biology). View Reference
View All (7) View Less
558036 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.