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Purified Mouse Anti-UBE3A
Purified Mouse Anti-UBE3A

Western blot analysis of UBE3A on a EB-1 cell lysate (Human B lymphoblast; Burkitt's lymphoma; ATCC HTB-60). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- UEB3A antibody.

Western blot analysis of UBE3A on a EB-1 cell lysate (Human B lymphoblast; Burkitt's lymphoma; ATCC HTB-60). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- UEB3A antibody.

Product Details
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BD Transduction Laboratories™
Ubiquitin Protein Ligase 3A
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Mouse UBE3A aa. 501-712
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
100 kDa
250 µg/ml
AB_398938
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611416 Rev. 1
Antibody Details
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13/UBE3A

Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. UBE3A (human papilloma virus E6-associated protein; E6-AP) is an E3 ubiquitin protein ligase that is linked to the neurological disorder Angelman syndrome. It mediates the interaction of human papilloma virus types 16 and 18 E6 proteins with p53, which targets p53 to the ubiquitination-proteasome degradation pathway. The C-terminal region of UBE3A contains a hect domain, which is found in many E3 ubiquitin protein ligases, while the central region contains an E6 binding region. UBE3A is expressed in the cytoplasm and nucleus of many tissues. The interaction of UBE3A with the progesterone receptor coactivates transciptional activity of the receptor in the presence of bound hormone. Thus, UBE3A is an essential component of the ubiquitination degradation pathway and the nuclear hormone receptor signal transduction pathways.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611416 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611416 Rev.1
Citations & References
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Development References (2)

  1. Matsuura T, Sutcliffe JS, Fang P. De novo truncating mutations in E6-AP ubiquitin-protein ligase gene (UBE3A) in Angelman syndrome. Nat Genet. 1997; 15(1):74-77. (Biology). View Reference
  2. Nawaz Z, Lonard DM, Smith CL. The Angelman syndrome-associated protein, E6-AP, is a coactivator for the nuclear hormone receptor superfamily. Mol Biol Cell. 1999; 19(2):1182-1189. (Biology). View Reference
611416 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.