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BD Transduction Laboratories™ Purified Mouse Anti-SRPK1
Clone 12/SRPK1 (RUO)
Western blot analysis of SRPK1 on HeLa cell lysate. Lane 1:1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-SRPK1.
Immunofluorescent staining of EaHy cells.
Western blot analysis of SRPK1 on HeLa cell lysate. Lane 1:1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-SRPK1.
Immunofluorescent staining of EaHy cells.
Product Details
BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human SRPK1 aa.312-434
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
92 kDa
250 µg/ml
AB_398385
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611072 Rev. 1
Antibody Details
12/SRPK1
Mammalian cell pre-mRNA splicing is mediated by the spliceosome, a multi-component complex that contains two types of splicing factors: small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP factors. Interactions between snRNPs and pre-mRNA ensures proper establishment of a catalytic core for the splicing reaction. However, these interactions are mediated by the non-snRNP factors. The super family of Arg/Ser-rich (RS) domain containing splicing factors are well known non-snRNPs. All of these proteins share a similar structure consisting of an N-terminal RNA recognition motif and a C-terminal RS domain. However, different SR factors have distinct specificities and function is regulated by their level of expression and by reversible phosphorylation. Two families of kinases phosphorylate SR domain-containing proteins: SR protein-specific kinases (SRPK1 and 2) and Clk/Sty. SRPL1 is specific for SR domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, whereas SRPK2 is highly expressed in brain. SRPKs affect splice-site selection and are thought to affect alternative splicing.
611072 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611072 Rev.1
Citations & References
View product citations for antibody "611072" on CiteAb
Development References (2)
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Colwill K, Feng LL, Yeakley JM. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem. 1996; 271(40):24569-24575. (Biology). View Reference
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Wang HY, Lin W, Dyck JA. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J Cell Biol. 1998; 140(4):737-750. (Biology). View Reference
611072 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.