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Purified Mouse Anti-Paxillin
Purified Mouse Anti-Paxillin

Western blot analysis of Paxillin of human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1: 2000, lane 3: 1: 4000 dilution of anti-Paxillin.

Purified Mouse Anti-Paxillin

Immunofluorescent staining of WI38 cells with anti-Paxillin.

Western blot analysis of Paxillin of human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1: 2000, lane 3: 1: 4000 dilution of anti-Paxillin.

Immunofluorescent staining of WI38 cells with anti-Paxillin.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Chicken Paxillin aa. 1-557
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
68 kDa
250 µg/ml
AB_397952
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610620 Rev. 1
Antibody Details
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165/Paxillin

Paxillin, a focal adhesion protein, is a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. This is consisent with the possibility that paxillin is involved in the regulation of cell morphology. Additionally, because of its SH3 binding domain, paxillin associates tightly with FAK and Crk in an extracellular matrix-independent manner. Paxillin was initially detected in fibroblasts, and its phosphorylation may be important during neurite extension during differentiation.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610620 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610620 Rev.1
Citations & References
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Development References (5)

  1. Herreros L, Rodriguez-Fernandez JL, Brown MC. Paxillin localizes to the lymphocyte microtubule organizing center and associates with the microtubule cytoskeleton. J Biol Chem. 1995; 270(34):5039-5047. (Clone-specific: Immunofluorescence). View Reference
  2. Laukaitis CM, Webb DJ, Donais K, Horwitz AF. Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. J Cell Biol. 2001; 153(7):1427-1440. (Clone-specific: Immunofluorescence). View Reference
  3. Muller G, Jung C, Wied S, Welte S, Jordan H, Frick W. Redistribution of glycolipid raft domain components induces insulin-mimetic signaling in rat adipocytes. Mol Cell Biol. 2001; 21(14):4553-4567. (Clone-specific: Western blot). View Reference
  4. Salgia R, Li JL, Lo SH, et al. Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. J Biol Chem. 1995; 270(10):5039-5047. (Biology). View Reference
  5. Turner CE, Glenney JR Jr, Burridge K. Paxillin: a new vinculin-binding protein present in focal adhesions. J Cell Biol. 1990; 111(3):1059-1068. (Biology). View Reference
View All (5) View Less
610620 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.