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Purified Mouse Anti-p150 [Glued]
Purified Mouse Anti-p150 [Glued]

Western blot analysis of p150 [Glued] on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- p150 [Glued] antibody.

Purified Mouse Anti-p150 [Glued]

Immunoflourescent staining of human endothelial cells.

Western blot analysis of p150 [Glued] on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- p150 [Glued] antibody.

Immunoflourescent staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Rat p150 [Glued] aa. 3-202
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-zinc-fixed, Immunoprecipitation (Tested During Development)
150 kDa
250 µg/ml
AB_397846
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610473 Rev. 1
Antibody Details
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1/p150Glued

p150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein, the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule bindings assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association.  

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610473 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610473 Rev.1
Citations & References
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Development References (5)

  1. Askham JM, Vaughan KT, Goodson HV, Morrison EE. Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome. Mol Biol Cell. 2002; 13(10):3627-3645. (Clone-specific: Immunofluorescence, Western blot). View Reference
  2. Aumais JP, Tunstead JR, McNeil RS, et al. NudC associates with Lis1 and the dynein motor at the leading pole of neurons. J Neurosci. 2001; 21(24):RC187:1-RC187:7. (Clone-specific: Immunofluorescence). View Reference
  3. King SJ, Schroer TA. Dynactin increases the processivity of the cytoplasmic dynein motor. Nat Cell Biol. 2000; 2(1):20-24. (Clone-specific). View Reference
  4. Kurzchalia T. Anthrax toxin rafts into cells. J Cell Biol. 2003; 160(3):295-296. (Clone-specific). View Reference
  5. Tai AW, Chuang JZ, Sung CH. Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport. J Cell Biol. 2001; 153(7):1499-1509. (Clone-specific: Immunofluorescence, Western blot). View Reference
View All (5) View Less
610473 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.