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Purified Mouse Anti- NFAT-1
Purified Mouse Anti- NFAT-1

Western blot analysis of NFAT-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti- NFAT-1 antibody. NFAT-1 may be identified migrating between 97-135 kDa.

Purified Mouse Anti- NFAT-1

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152).

Western blot analysis of NFAT-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti- NFAT-1 antibody. NFAT-1 may be identified migrating between 97-135 kDa.

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152).

Product Details
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BD Transduction Laboratories™
NF-ATc2; NFATP; KIAA0611
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human NFAT-1 aa. 29-181
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development)
97-135 kDa
250 µg/ml
AB_398025
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610702 Rev. 2
Antibody Details
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1/NFAT-1

T cells are activated and induced to proliferate following binding of their respective antigen. The process of includes expression of genes that encode factors (i.e., cytokines) which regulate various cell types. Modulation of gene expression is conducted by an array of specific interactions between transcription factors and DNA. NFAT-1 (Nuclear Factor of Activated T cells) is a transcription factor that regulates expression of the interleukin-2 gene. Thus, NFAT-1 DNA binding activity is undetectable in resting cells, but increases during T-cell activation. NFAT-1, a protein of 921 amino acids, is part of an oligomeric transcription factor that also contains Fra-1 and JunB. NFAT-1 was initially described as a phosphoprotein and is dephosphorylated in activated T cells transformed with the leukemia virus HTLV-l.

610702 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610702 Rev.2
Citations & References
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Development References (5)

  1. Boise LH, Petryniak B, Mao X, et al. The NFAT-1 DNA binding complex in activated T cells contains Fra-1 and JunB. Mol Cell Biol. 1993; 13(3):1911-1919. (Biology). View Reference
  2. Good L, Maggirwar SB, Harhaj EW, Sun SC. Constitutive dephosphorylation and activation of a member of the nuclear factor of activated T cells, NF-AT1, in Tax-expressing and type I human T-cell leukemia virus-infected human T cells. J Biol Chem. 1997; 272(3):1425-1428. (Biology). View Reference
  3. Grader-Beck T, van Puijenbroek AA, Nadler LM, Boussiotis VA. cAMP inhibits both Ras and Rap1 activation in primary human T lymphocytes, but only Ras inhibition correlates with blockade of cell cycle progression. Blood. 2003; 101(3):998-1006. (Biology: Western blot). View Reference
  4. Kadereit S, Mohammad SF, Miller RE, et al. Reduced NFAT1 protein expression in human umbilical cord blood T lymphocytes. Blood. 1999; 94(9):3101-3107. (Biology: Western blot). View Reference
  5. McCaffrey PG, Luo C, Kerppola TK, et al. Isolation of the cyclosporin-sensitive T cell transcription factor NFATp. Science. 1993; 262(5134):750-754. (Biology). View Reference
View All (5) View Less
610702 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.