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Purified Mouse Anti-NAT1
Purified Mouse Anti-NAT1

Western blot analysis of NAT1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-NAT1 antibody.

  

Purified Mouse Anti-NAT1

Immunofluorescence staining of mouse macrophages.

Western blot analysis of NAT1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-NAT1 antibody.

  

Immunofluorescence staining of mouse macrophages.

Product Details
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BD Transduction Laboratories™
Novel APOBEC-1 Target no. 1; DAP-5; Death Associated Protein -5
Human (QC Testing), Mouse, Rat, Dog, Cow (Tested in Development)
Mouse IgG1
Human NAT1 aa. 672-830
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
100 kDa
250 µg/ml
AB_398066
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610742 Rev. 1
Antibody Details
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35/NAT1

eIF-4 proteins are required for recognition of mRNA and acceleration of protein translation. This group of proteins consists of the RNA helicase eIF-4A, the RNA-binding protein eIF-4B, the cap-binding protein eIF-4E, and eIF-4G (p220).  NAT1 (Novel APOBEC-1 Target no. 1), also known as DAP-5 (Death Associated Protein -5), is homologous to eIF-4G. Amino acid sequence comparison of NAT1 and eIF-4 shows that NAT1 lacks an eIF-4G N-terminal region. This region mediates eIF-4G association with eIF-4E. The highest degree of homology is within the central portions of NAT1 and eIF-4G, while the lowest degree of homology occurs at the C-terminus. NAT1 more closely resembles a cleaved form of eIF-4G that is involved in cap-independent translation. It is thought that NAT1 is involved in the repression of translation via its inhibition of both cap-dependent and cap-independent translation.

610742 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610742 Rev.1
Citations & References
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Development References (3)

  1. Henis-Korenblit S, Strumpf NL, Goldstaub D, Kimchi A. A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation. Mol Cell Biol. 2000; 20(2):496-506. (Biology: Western blot). View Reference
  2. Levy-Strumpf N, Deiss LP, Berissi H, Kimchi A. DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death. Mol Cell Biol. 1997; 17(3):1615-1625. (Biology). View Reference
  3. Yamanaka S, Poksay KS, Arnold KS, Innerarity TL. A novel translational repressor mRNA is edited extensively in livers containing tumors caused by the transgene expression of the apoB mRNA-editing enzyme. Genes Dev. 1997; 11(3):321-333. (Biology). View Reference
610742 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.