Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Norway (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-Human Nup88
      Purified Mouse Anti-Human Nup88
      Western blot analysis of Nup88 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the Mouse Anti-Human Nup88 antibody.
      Purified Mouse Anti-Human Nup88
      Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
      Purified Mouse Anti-Human Nup88 Purified Mouse Anti-Human Nup88
      Western blot analysis of Nup88 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the Mouse Anti-Human Nup88 antibody.
      Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
      Product Details
      Down Arrow Up Arrow


      BD Transduction Laboratories™
      Human (QC Testing)
      Mouse IgG1
      Human Nup88 aa. 314-425
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
      88 kDa
      250 µg/ml
      AB_399376
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      611896 Rev. 2
      Antibody Details
      Down Arrow Up Arrow
      22/Nup88

      Nucleocytoplasmic transport involves the binding and interaction of several cytosolic and nuclear pore proteins by at least two different mechanisms. One mechanism is mediated by karyopherins to transport proteins with the nuclear localization sequence (NLS) and an alternative pathway uses the protein Transportin. Morphologically both pathways require the use of the nuclear pore complex (NPC) which acts as a gate mediating active transport of proteins and RNA into and out of the nucleus. The NLS binds to karyopherin α, and to the N-terminal region of karyopherin β. Both karyopherins bind to repeat sequences of nucleoporins at the nuclear envelope. p62 is the best characterized member of a group of nucleoporins that line the central region of the NPC. A tightly associated complex is formed by p62 and two other nucleoporins, p54 and p58.  Nup88 is a nonglycosylated nucleoporin, that does not contain the characteristic GLFG or XFXFG repeats found in other NPC proteins. The structure of Nup88 includes a coiled-coil domain required for association with the NPC. Nup88 is overexpressed during oncogenesis and development, and forms a complex with the nuclear transport factors, Exportin-1 and Nup214.

      611896 Rev. 2
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      611896 Rev.2
      Citations & References
      Down Arrow Up Arrow

      Development References (3)

      1. Bastos R, Ribas de Pouplana L, Enarson M, Bodoor K, Burke B. Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex. J Cell Biol. 1997; 137(5):989-1000. (Biology). View Reference
      2. Fornerod M, van Deursen J, van Baal S. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88. EMBO J. 1997; 16(4):807-816. (Biology). View Reference
      3. Martinez N, Alonso A, Moragues MD, Ponton J, Schneider J. The nuclear pore complex protein Nup88 is overexpressed in tumor cells. Cancer Res. 1999; 59(21):5408-5411. (Biology). View Reference
      611896 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback