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Purified Mouse Anti-Human hCNK1
Purified Mouse Anti-Human hCNK1

Western blot analysis of hCNK1 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human hCNK1 antibody. hCNK1 has a calculated molecular weight of 79 kDa, but may be observable migrating at ~ 100 kDa.

Purified Mouse Anti-Human hCNK1

Immunofluorescence staining of human endothelial cells.

Western blot analysis of hCNK1 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human hCNK1 antibody. hCNK1 has a calculated molecular weight of 79 kDa, but may be observable migrating at ~ 100 kDa.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Connector enhancer of KSR
Human (QC Testing)
Mouse IgG1
Human hCNK1 aa. 10-217
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
79-100 kDa
250 µg/ml
AB_399210
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611734 Rev. 1
Antibody Details
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46/hCNK1

Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. These proteins relay signals from Tyr-kinases at the plasma membrane to the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway. Kinase suppressor of Ras (KSR-1) was discovered in Drosophila in a genetic screen that identified mutations that suppress constitutively active Ras mutants. Connector enhancer of KSR (CNK) was found in a similar screen to identify mutations that enhance the KSR- dependent phenotype. Drosophila CNK contains a sterile alpha motif (SAM), a conserved region in CNK (CRIC), and a PDZ domain in the N-terminal region, a proline-rich and a plecstrin homology (PH) domain in the central region, and a C-terminal Pro-rich domain. The human homologue of CNK (hCNK1) contains similar N-terminal and central domains, but is 713 amino acids in length compared to 1557 amino acids for Drosophila CNK. In Drosophila, the N-terminal region of CNK facilitates binding to RAS, while the C-terminal region inhibits RAS- and RAF-dependent signaling. Thus, CNK may be important for regulation of both RAS- and RAF-dependent signaling.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (2)

  1. Therrien M, Wong AM, Kwan E, Rubin GM. Functional analysis of CNK in RAS signaling. Cell. 1999; 96(23):13259-13263. (Biology). View Reference
  2. Therrien M, Wong AM, Rubin GM. CNK, a RAF-binding multidomain protein required for RAS signaling. Cell. 1998; 95(3):343-353. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.