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Purified Mouse Anti-Human CD104
Purified Mouse Anti-Human CD104

Western blot analysis of CD104 (Integrin β4) on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human CD104 antibody.

Purified Mouse Anti-Human CD104

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of CD104 (Integrin β4) on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human CD104 antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
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BD Transduction Laboratories™
Integrin β4
Human (QC Testing)
Mouse IgG1
Human Integrin β4 aa. 1612-1821
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
200 kDa
250 µg/ml
AB_398763
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611232 Rev. 2
Antibody Details
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7/CD104

Cell adhesion to extracellular matrix components or to cell surface proteins, especially those expressed by leukocytes and endothelial cells, is mediated by integrins. Integrins contain noncovalently associated α and β subunits. At least 17 α and 8 β subunits have been identified and these proteins can heterodimerize to form 22 different receptors. The α6β4 integrin is a receptor for various laminins and binds with the highest affinity to laminins 4 and 5. It exhibits elevated expression in the basal cell layer of stratified epithelia, in Schwann cells at the onset of myelination, and in CD4-CD8- pre-T lymphocytes entering the thymus. In addition, α6β4 expression is increased in squamous carcinomas where it promotes invasion through a targeting of PI3 kinase activity. The majority of β4 comprises a cytoplasmic domain with unique signaling properties. The C-terminal portion of this domain contains two pairs of type III fibronectin-like motifs (FNIII) and a tyrosine activation motif (TAM). Additional domains in the cytoplasmic tail bind Shc and activate the MAPK pathway. Thus, integrin β4 is an integrin subunit that is important for cell survival, growth, and differentiation.

611232 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611232 Rev.2
Citations & References
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Development References (2)

  1. Mainiero F, Pepe A, Wary KK, et al. Signal transduction by the alpha 6 beta 4 integrin: distinct beta 4 subunit sites mediate recruitment of Shc/Grb2 and association with the cytoskeleton of hemidesmosomes. EMBO J. 1995; 14(18):4470-4481. (Biology). View Reference
  2. Shaw LM, Rabinovitz I, Wang HH, Toker A, Mercurio AM. Activation of phosphoinositide 3-OH kinase by the alpha6beta4 integrin promotes carcinoma invasion. Cell. 1997; 91(7):949-960. (Biology). View Reference
611232 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.