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Purified Mouse Anti-HAX-1
Purified Mouse Anti-HAX-1

Western blot analysis of HAX-1 on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-HAX-1 antibody.

Purified Mouse Anti-HAX-1

Immunofluorescent staining of HeLa cells with anti-HAX-1 antibody.

Western blot analysis of HAX-1 on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-HAX-1 antibody.

Immunofluorescent staining of HeLa cells with anti-HAX-1 antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human HAX-1 aa. 10-148
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
35 kDa
250 µg/ml
AB_398143
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610824 Rev. 1
Antibody Details
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52/HAX-1

Cell-cell interactions in the immune system, mediated by membrane-bound antigen receptors, trigger a cascade of signaling mechanisms collectively known as the T-cell response. Upon antigen binding, cellular tyrosine kinases are activated resulting in the phosphorylation of HS1 or Lck-binding protein 1 (LckBP1), which is involved in thymic negative selection. HS1 is one of the earliest intracellular proteins to be tyrosine phosphorylated in response to antigen-receptor cross-linking in T cells. HAX-1 (HS1-associated protein X-1) is a 35 kDa protein found associated with HS1 by co-immunoprecipitation assays and the two-hybrid system. HAX-1 is ubiquitously expressed and localized in the mitochondria, endoplasmic reticulum, and the nuclear envelope. The HS1-HAX-1 complex may be critical for signal transduction pathways initiated by antigen presentation to T cells.

610824 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610824 Rev.1
Citations & References
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Development References (1)

  1. Suzuki Y, Demoliere C, Kitamura D, Takeshita H, Deuschle U, Watanabe T. HAX-1, a novel intracellular protein, localized on mitochondria, directly associates with HS1, a substrate of Src family tyrosine kinases. J Immunol. 1997; 158(6):2736-2744. (Biology). View Reference
610824 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.