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Purified Mouse Anti-GM130
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1, κ
Rat GM130 aa. 869-982
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
130 kDa
250 µg/ml
AB_398142
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610823 Rev. 1
Antibody Details
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35/GM130

Maturation and post-translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. The detergent insoluble fraction of the Golgi is named "matrix" and is required for proper morphology of the Golgi membranes. GM130 (Golgi matrix protein of 130 kDa) is a protein isolated from the Triton™ X-100-insoluble Golgi matrix and peripherally associated with the cis-compartment, as demonstrated by co-localization with syntaxin5. GM130 is homologous to the Golgi autoantigen golgin 95. GM130 interacts through its N-terminal domain with p115 and with the Golgi membranes at the C-terminal portion. Furthermore, the mitotic phosphorylation of GM130 blocks the interaction with p115. Thus, GM130 appears to function as a structural element of the Golgi apparatus that also provides attachment sites for membranes and other Golgi proteins.  The 35/GM130 monoclonal antibody recognizes GM130, regardless of phosphorylation status.

610823 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610823 Rev.1
Citations & References
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Development References (5)

  1. Ireton RC, Davis MA, van Hengel J, et al. A novel role for p120 catenin in E-cadherin function. J Cell Biol. 2002; 159(3):465-476. (Clone-specific: Western blot). View Reference
  2. Marra P, Maffucci T, Daniele T, et al. The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment. Nat Cell Biol. 2001; 3(12):1101-1113. (Clone-specific: Immunofluorescence). View Reference
  3. Nakamura N, Lowe M, Levine TP, Rabouille C, Warren G. The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner. Cell. 1997; 89(3):445-455. (Biology). View Reference
  4. Perez F, Pernet-Gallay K, Nizak C, Goodson HV, Kreis TE, Goud B. CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family. J Cell Biol. 2002; 156(4):631-642. (Clone-specific: Immunofluorescence). View Reference
  5. Ralston E, Lu Z, Ploug T. The organization of the Golgi complex and microtubules in skeletal muscle is fiber type-dependent. J Neurosci. 1999; 19(24):10694-10705. (Clone-specific: Immunohistochemistry). View Reference
View All (5) View Less
610823 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.