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Purified Mouse Anti-Drp1
Purified Mouse Anti-Drp1

Western blot analysis of Drp1 on a HCT-8 cell lysate (Human colorectal adenocarcinoma; ATCC CCL-244). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Drp1 antibody.

Purified Mouse Anti-Drp1

Immunofluorescence staining on A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of Drp1 on a HCT-8 cell lysate (Human colorectal adenocarcinoma; ATCC CCL-244). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Drp1 antibody.

Immunofluorescence staining on A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
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BD Transduction Laboratories™
Density Regulated Protein-1
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG2a
Human Drp1 aa. 19-201
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
27 kDa
250 µg/ml
AB_399215
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611738 Rev. 1
Antibody Details
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22/Drp1

In culture, cell density can have profound effects on gene expression, enzymatic activity, and cell signaling pathways. Using differential screening of cDNAs from low-passage nontumorigenic teratocarcinoma cells versus high passage tumorigenic cells, a protein was identified that is regulated in cell-density dependent manner. This protein, density-regulated protein 1 (drp1), contains putative sites for N-myristoylation and phosphorylation sites for cAMP and/or cGMP-dependent kinase, casein kinase II, and PKC. The expression of drp1 is enriched in high density cultures of both nontumorigenic and tumorigenic cell lines and is widely detected in adult organs, especially skeletal and cardiac muscle. In addition, increased expression of drp1 is not due to growth arrest as a result of serum starvation or TGF-β treatment nor is it a result of factors found in the media of high density cultures. Interestingly, drp1 is expressed highest in skeletal and cardiac muscle where unique cell-cell contacts are involved in muscle cell membrane depolarization and contraction. Thus, drp1 expression may be regulated by signaling pathways related to specific types of cell-cell contacts.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611738 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611738 Rev.1
Citations & References
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Development References (1)

  1. Deyo JE, Chiao PJ, Tainsky MA. drp, a novel protein expressed at high cell density but not during growth arrest. DNA Cell Biol. 1998; 17(5):437-447. (Biology). View Reference
611738 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.