Preparation And Storage
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The human homologue of the Drosophila discs large tumor suppressor protein (hDlg) is a member of the MAGUKs (membrane associated guanylate kinases) protein family. Members of this family (PSD-95, ZO-1, ZO-2, and human erythroid p55) are involved in cell structure and signaling events. The hDlg protein consists of several domains: three PDZ (PSD-95/Discs large/ZO-1) domains, an SH3 domain, and a guanylate kinase-like domain. However, hDlg contains a proline rich N-terminus region consisting of two SH3 domain binding sites that are not normally found in the MAGUKs family. The PDZ domains mediate the interaction of several proteins, such as Shaker-type K+ channel proteins and the APC tumor suppressor protein. Dlg is a peripheral membrane that associates with the cytoskeleton. The cellular location and binding sites of Dlg suggest a role in structure, signal transduction, and growth regulation. Supporting these probable Dlg functions are reports demonstrating that recessive mutations in Drosophila dlg lead to imaginal disc neoplasia and death. Also, Dlg has been reported to bind p56 [lck] tyrosine kinase and the Kv1.3 channel in human T lymphocytes.
This antibody clone to Dlg has been reported to immunoprecipitate and weakly recognize PSD-95 on rat cerebrum lysates, presumably due to crossreactivity between homologous regions of the two proteins (i.e amino acid regions 104-119 and 193-198).
Development References (5)
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Adey NB, Huang L, Ormonde PA, et al. Threonine phosphorylation of the MMAC1/PTEN PDZ binding domain both inhibits and stimulates PDZ binding. Cancer Res. 2000; 60(1):35-37. (Biology: Western blot). View Reference
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Caruana G, Bernstein A. Craniofacial dysmorphogenesis including cleft palate in mice with an insertional mutation in the discs large gene. Mol Cell Biol. 2001; 21(5):1475-1483. (Biology: Immunofluorescence, Western blot). View Reference
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Lue RA, Brandin E, Chan EP, Branton D. Two independent domains of hDlg are sufficient for subcellular targeting: the PDZ1-2 conformational unit and an alternatively spliced domain. J Cell Biol. 1996; 135(4):1125-1137. (Biology). View Reference
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Lue RA, Marfatia SM, Branton D, Chishti AH. Cloning and characterization of hdlg: the human homologue of the Drosophila discs large tumor suppressor binds to protein 4.1. Proc Natl Acad Sci U S A. 1994; 91(21):9818-9822. (Biology). View Reference
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Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers WR. Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex. J Biol Chem. 2001; 276(52):48627-48630. (Biology: Western blot). View Reference
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