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Western blot anakysis of Chk2 on RSV-3T3 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Chk2.
BD Transduction Laboratories™ Purified Mouse Anti-Chk2
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The cell cycle is regulated by multiple checkpoints that determine cell fate. Such checkpoints ensure that DNA replication and chromosomal segregation are completed with high fidelity. When DNA is damaged, specific kinases and phosphatases, key components of cell cycle checkpoints, function to arrest the cell cycle and provide the necessary time for DNA repair. For example, DNA damage induces arrest of the cell cycle at the G2 checkpoint via Wee1-mediated inhibitory phosphorylation of the kinase Cdc2. Inhibition is relieved by the Cdc25C phosphatase via Cdc2 dephosphorylation. In turn, the Chk1 and Chk2 protein kinases phosphorylate and inhibit Cdc25C, thus preventing activation of the Cdc2-cyclin B complex and entry into mitosis. Chk2, the human homolog of S. cerevisiae Rad53 and S. pombe Cds1, contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA) which is found in other cell cycle kinases. Chk2 is expressed during late G1 to M phase and is found in the nucleoplasm. Thus, Chk1 and Chk2 activity may be an important checkpoint that inhibits entry into mitosis in response to DNA damage.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (2)
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Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998; 282(5395):1893-1897. (Biology). View Reference
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Tominaga K, Morisaki H, Kaneko Y, et al. Role of human Cds1 (Chk2) kinase in DNA damage checkpoint and its regulation by p53. J Biol Chem. 1999; 274(44):31463-31467. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.