Skip to main content Skip to navigation
Purified Mouse Anti-Calcineurin
Purified Mouse Anti-Calcineurin
Western blot analysis of Calcineurin on a rat cerebrum lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Calcineurin antibody.
Purified Mouse Anti-Calcineurin
Immunohistochemical staining of a rabbit brain section.
Purified Mouse Anti-Calcineurin
Immunohistochemical staining of pyrimidal cells in the rat hippocampus, formalin-fixed paraffin-embedded tissue section with no pre-treatment (20X magnification).
Western blot analysis of Calcineurin on a rat cerebrum lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Calcineurin antibody.
Immunohistochemical staining of a rabbit brain section.
Immunohistochemical staining of pyrimidal cells in the rat hippocampus, formalin-fixed paraffin-embedded tissue section with no pre-treatment (20X magnification).
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Chicken, Frog (Tested in Development)
Mouse IgG2a
Human Calcineurin aa. 247-449
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
61 kDa
250 µg/ml
AB_397655
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610259 Rev. 14
Antibody Details
Down Arrow Up Arrow
29/Calcineurin

Calcineurin is a Ca2+/calmodulin-dependent protein phosphatase. The active enzyme is a heterodimer of a large calmodulin-binding catalytic subunit A (61 kDa) and a smaller Ca2+ binding subunit B (19 kDa). Regions corresponding to the calmodulin-binding site, an autoinhibitory domain, and a putative subunit B binding site have been identified within the large subunit A. The activity of calcineurin is sensitive to immunosuppressants such as cyclosporin A (CsA) and tacrolimus (FK506). The study of FK506-mediated inhibition of nitric oxide formation has revealed that nitric oxide synthase (NOS) is a calcineurin substrate. Calcineurin dephosphorylates NOS and enhances its catalytic activity. Therefore, Calcineurin is an essential mediator for efficient T cell antigen receptor (TCR)-mediated T cell activation.

610259 Rev. 14
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610259 Rev.14
Citations & References
Down Arrow Up Arrow
View product citations for antibody "610259" on CiteAb

Development References (5)

  1. Jicha GA, Weaver C, Lane E. cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease. J Neurosci. 1999; 19(17):7486-7494. (Biology: Western blot). View Reference
  2. Liang H, Venema VJ, Wang X, Ju H, Venema RC, Marrero MB. Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells. J Biol Chem. 1999; 274(28):19846-19851. (Biology: Immunoprecipitation, Western blot). View Reference
  3. Sik A, Hajos N, Gulacsi A, Mody I, Freund TF. The absence of a major Ca2+ signaling pathway in GABAergic neurons of the hippocampus. Proc Natl Acad Sci U S A. 1998; 95(96):3245-3250. (Biology: Immunohistochemistry). View Reference
  4. Vega RB, Rothermel BA, Weinheimer CJ. Dual roles of modulatory calcineurin-interacting protein 1 in cardiac hypertrophy. Proc Natl Acad Sci U S A. 2003; 100(2):669-674. (Biology: Western blot). View Reference
  5. Verin AD, Cooke C, Herenyiova M, Patterson CE, Garcia JG. Role of Ca2+/calmodulin-dependent phosphatase 2B in thrombin-induced endothelial cell contractile responses. Am J Physiol. 1998; 275(4 pt 1):L788-L799. (Biology: Immunofluorescence, Western blot). View Reference
View All (5) View Less
610259 Rev. 14

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.