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Western blot analysis of Calcineurin on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Calcineurin antibody.
Immunohistochemical staining of a rabbit brain section.
Immunohistochemical staining of pyrimidal cells in the rat hippocampus, formalin-fixed paraffin-embedded tissue section with no pre-treatment (20X magnification).
BD Transduction Laboratories™ Purified Mouse Anti-Calcineurin
BD Transduction Laboratories™ Purified Mouse Anti-Calcineurin
BD Transduction Laboratories™ Purified Mouse Anti-Calcineurin
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Calcineurin is a Ca2+/calmodulin-dependent protein phosphatase. The active enzyme is a heterodimer of a large calmodulin-binding catalytic subunit A (61 kDa) and a smaller Ca2+ binding subunit B (19 kDa). Regions corresponding to the calmodulin-binding site, an autoinhibitory domain, and a putative subunit B binding site have been identified within the large subunit A. The activity of calcineurin is sensitive to immunosuppressants such as cyclosporin A (CsA) and tacrolimus (FK506). The study of FK506-mediated inhibition of nitric oxide formation has revealed that nitric oxide synthase (NOS) is a calcineurin substrate. Calcineurin dephosphorylates NOS and enhances its catalytic activity. Therefore, Calcineurin is an essential mediator for efficient T cell antigen receptor (TCR)-mediated T cell activation.
Development References (5)
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Jicha GA, Weaver C, Lane E. cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease. J Neurosci. 1999; 19(17):7486-7494. (Biology: Western blot). View Reference
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Liang H, Venema VJ, Wang X, Ju H, Venema RC, Marrero MB. Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells. J Biol Chem. 1999; 274(28):19846-19851. (Biology: Immunoprecipitation, Western blot). View Reference
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Sik A, Hajos N, Gulacsi A, Mody I, Freund TF. The absence of a major Ca2+ signaling pathway in GABAergic neurons of the hippocampus. Proc Natl Acad Sci U S A. 1998; 95(96):3245-3250. (Biology: Immunohistochemistry). View Reference
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Vega RB, Rothermel BA, Weinheimer CJ. Dual roles of modulatory calcineurin-interacting protein 1 in cardiac hypertrophy. Proc Natl Acad Sci U S A. 2003; 100(2):669-674. (Biology: Western blot). View Reference
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Verin AD, Cooke C, Herenyiova M, Patterson CE, Garcia JG. Role of Ca2+/calmodulin-dependent phosphatase 2B in thrombin-induced endothelial cell contractile responses. Am J Physiol. 1998; 275(4 pt 1):L788-L799. (Biology: Immunofluorescence, Western blot). View Reference
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