Skip to main content Skip to navigation
Purified Mouse Anti-BRUCE
Purified Mouse Anti-BRUCE

Western blot analysis of BRUCE on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-BRUCE antibody.

Purified Mouse Anti-BRUCE

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of BRUCE on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-BRUCE antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
BIR Repeat containing Ubiquitin-Conjugating Enzyme
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Mouse BRUCE aa. 372-571
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
528 kDa
250 µg/ml
AB_398727
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp

Clone 4/BRUCE has also been shown to work well for Western blot application on Hela lysate (Cat. No. 611449)

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611193 Rev. 1
Antibody Details
Down Arrow Up Arrow
4/BRUCE

Selective proteolysis is essential for the modulation of key cellular processes such as cell cycle progression.  However, unlike other post-translational events, proteolysis is irreversible and therefore must occur in unidirectional cellular pathways.  In eukaryotes, proteolysis is mediated primarily by the ubiquitin pathway.  This pathway designates proteins for degradation by the proteasome, a multicatalytic protease complex.  The ubiquitin pathway is a multistep system that tags proteins for degradation via the attachment of ubiquitin molecules to the target.  This attachment is mediated by the ubiquitin activating/conjugating enzymes E1, E2, E3, and BRUCE.  BRUCE (BIR Repeat containing Ubiquitin-Conjugating Enzyme) is a novel enzyme that associates with the Golgi and the vesicular system.  It contains a UBC (ubiquitin conjugating enzyme) domain, which is essential for catalysis, and a BIR (baculovirus inhibitor of apoptosis repeat) motif.  BIR motifs are also found within inhibitor of apoptosis proteins (IAP) and are critical for anti-apoptotic activity.  Therefore, BRUCE may function to both mediate ubiquitin-dependent proteolysis and contribute to anti-apoptotic cellular pathways.

611193 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611193 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (1)

  1. Hauser HP, Bardroff M, Pyrowolakis G, Jentsch S. A giant ubiquitin-conjugating enzyme related to IAP apoptosis inhibitors. J Cell Biol. 1998; 141(6):1415-1422. (Biology). View Reference
611193 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.