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Purified Mouse Anti-Bax
Purified Mouse Anti-Bax

Western blot analysis of Bax on a HepG2 cell lysate (Human hepatocellular carcinoma; ATCC HB-8065). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Bax antibody.

Purified Mouse Anti-Bax

Immunofluorescence staining of BC3H1 cells (Mouse brain smooth muscle-like cells; ATCC CRL-1443).

Western blot analysis of Bax on a HepG2 cell lysate (Human hepatocellular carcinoma; ATCC HB-8065). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Bax antibody.

Immunofluorescence staining of BC3H1 cells (Mouse brain smooth muscle-like cells; ATCC CRL-1443).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human Bax-α aa. 55-178
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development)
21 kDa
250 µg/ml
AB_398295
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610983 Rev. 1
Antibody Details
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3/Bax

Proper development and maintenance of homeostasis within tissues is achieved through cell proliferation, differentiation, and apoptosis. Members of the Bcl-2 protein family function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bak) apoptosis. Similar to other family members, Bax contains the conserved Bcl-2 homology 1 (BH1) and 2 (BH2) domains which allow for its homodimerization or heterodimerization with Bcl-2. Although Bax alone does not induce cell death, elevated levels of Bax accelerate apoptosis following a death signal such as cytokine deprivation or Fas/Fas ligand interaction. The ratio of Bax homodimers to Bax/Bcl-2 heterodimers is an apoptotic checkpoint. When Bcl-2 is in excess, apoptosis is inhibited. However, if Bax levels increase in response to a death signal, the cell is pushed toward death. Bax function is also affected by Bax Inhibitor-1 (BI-1). Although it interacts directly with Bcl-2 and not Bax, BI-1 can suppress Bax-induced apoptosis. Alternative splicing of Bax mRNA produces Bax-α, the integral membrane form, and the two cytosolic forms β and γ. These isoforms are thought to allow for differential regulation of Bax activity and localization.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610983 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610983 Rev.1
Citations & References
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Development References (5)

  1. Aoudjit F, Vuori K. Integrin signaling inhibits paclitaxel-induced apoptosis in breast cancer cells. Oncogene. 2001; 20(36):4995-5004. (Clone-specific: Western blot). View Reference
  2. Kartasheva NN, Contente A, Lenz-Stoppler C, Roth J, Dobbelstein M. p53 induces the expression of its antagonist p73 Delta N, establishing an autoregulatory feedback loop. Oncogene. 2002; 21(31):4715-4727. (Clone-specific: Western blot). View Reference
  3. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993; 74(4):609-619. (Biology). View Reference
  4. Rytomaa M, Lehmann K, Downward J. Matrix detachment induces caspase-dependent cytochrome c release from mitochondria: inhibition by PKB/Akt but not Raf signalling. Oncogene. 2000; 19(39):4461-4468. (Clone-specific: Western blot). View Reference
  5. Xu Q, Reed JC. Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast. Mol Cell. 1998; 1(3):337-346. (Biology). View Reference
View All (5) View Less
610983 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.