Purified Mouse Anti-Rat IL-10

BD Pharmingen™ Purified Mouse Anti-Rat IL-10

Name: Purified Mouse Anti-Rat IL-10
Brand: BD Pharmingen™
SKU: 555083

Clone A5-7

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Rat (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: E. coli-expressed rat IL-10

Application: ELISA Capture (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_395689

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified A5-7 antibody can be used as the capture antibody in a sandwich ELISA for measuring rat IL-10 protein levels in conjunction with the biotinylated A5-6 antibody (Cat. # 555084) as the detecting antibody and recombinant rat IL-10 (Cat. #555113) as the standard. This purified capture antibody should be pretitrated in the range of 1 to 4 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of rat IL-10 ranging from 2,000 to 5 pg/ml should be used. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring Rat IL-10 in serum or plasma, our BD OptEIA™ Rat IL-10 ELISA Set (Cat. No. 555134) is specially formulated and recommended.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

555083 Rev. 1

Antibody Details

A5-7

The A5-7 antibody reacts with rat Interleukin-10 (IL-10). The immunogen used to generate this hybridoma was E. coli-expressed rat IL-10.

555083 Rev. 1

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

555083 Rev.1

Citations & References

View product citations for antibody "555083" on CiteAb

Development References (1)

Feng L, Tang WW, Chang JC, Wilson CB. Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages. Biochem Biophys Res Commun. 1993; 192(2):452-458. (Immunogen). View Reference

555083 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.