Preparation And Storage
Recommended Assay Procedures
ELISA Detection: The biotinylated B4 antibody (Cat. No. 555074) can be used as the detection antibody in a sandwich ELISA for measuring rat MCP-1 protein levels in conjunction with purified C4 antibody (Cat. No. 555072) as the capture antibody and recombinant rat MCP-1 protein (Cat. No. 555110) as the standard. This biotinylated detection antibody should be titrated in the range of 0.5 to 2 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of rat MCP-1 protein ranging from ~2,000 to 5 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. This ELISA pair shows no cross-reactivity with rat MIP-1α, MIP-1β, RANTES, MIP-2 or KC.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not
recommended for assaying serum or plasma samples. For measuring MCP-1 in serum or plasma the rat MCP-1 BD OptEIA™ ELISA Set, Cat.
No. 555130, is specially formulated and recommended.
IP: The B4 antibody has been reported to be useful for immunoprecipitation studies. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The B4 antibody reacts with rat Monocyte Chemoattractant Protein 1 (MCP-1). The immunogen used to generate this hybridoma was recombinant rat MCP-1 protein.
Development References (1)
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Sakanashi Y, Takeya M, Yoshimura T, Feng L, Morioka T, Takahashi K. Kinetics of macrophage subpopulations and expression of monocyte chemoattractant protein-1 (MCP-1) in bleomycin-induced lung injury of rats studied by a novel monoclonal antibody against rat MCP-1. J Leukoc Biol. 1994; 56(6):741-750. (Clone-specific: ELISA, Immunoprecipitation). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.