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Human IFN-γ Enhanced Sensitivity Flex Set

Human IFN-γ Enhanced Sensitivity Flex Set

(RUO)
Human IFN-γ Enhanced Sensitivity Flex Set

Figure 1. Example BD CBA Human IFN-γ Enhanced Sensitivity Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Human IFN-γ Enhanced Sensitivity Flex Set

Linearity: Cell culture supernatant, 1:3 diluted serum, or 1:3 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Human IFN-γ Enhanced Sensitivity Flex Set

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human IFN-γ Enhanced Sensitivity Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay) respectively.

Human IFN-γ Enhanced Sensitivity Flex Set

Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:3 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.

Figure 1. Example BD CBA Human IFN-γ Enhanced Sensitivity Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Linearity: Cell culture supernatant, 1:3 diluted serum, or 1:3 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human IFN-γ Enhanced Sensitivity Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay) respectively.

Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:3 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.

Product Details
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BD™ Cytometric Bead Array (CBA)
B8
274-200,000 fg/mL
Human (QC Testing)
RUO
AB_2869377


Description

The BD™ CBA Human IFN-γ Enhanced Sensitivity Flex Set is a bead-based immunoassay capable of measuring human Interferon-γ (IFN-γ) in serum, plasma, and cell culture supernatant samples. The BD CBA Enhanced Sensitivity Flex Sets are capable of measuring soluble analytes present in very low concentrations. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Enhanced Sensitivity Master Buffer Kit (Cat. No. 561521 or 561523).

Preparation And Storage

This BD CBA Enhanced Sensitivity Flex Set contains one vial each of Enhanced Sensitivity Capture Bead and Detection Reagent (Part A) and two vials of Standard. The Enhanced Sensitivity Detection Reagent (Part B) is provided in the BD CBA Human Enhanced Sensitivity Master Buffer Kit. The Enhanced Sensitivity Capture Bead and Detection Reagent (Part A) components of this flex set have been formulated to a 20x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and when reconstituted according to the instructions in the BD CBA Human Enhanced Sensitivity Master Buffer kit, the top standard point for this assay is 200,000 fg/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the Detection Reagent from prolonged exposure to light.

Recommended Assay Procedures

The BD CBA Human IFN-γ Enhanced Sensitivity Flex Set must be used in conjunction with a BD CBA Human Enhanced Sensitivity Master Buffer Kit (Cat. No. 561521, 100 tests, or 561523, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Enhanced Sensitivity Master Buffer Kit. When following the directions in the Master Buffer Kit, the standard range for the BD CBA Human IFN-γ Enhanced Sensitivity Flex Set will be 274 to 200,000 fg/mL. An example standard curve is shown in Figure 1.

The BD CBA Human IFN-γ Enhanced Sensitivity Flex Set should only be used in the same assay well with other BD CBA Human Enhanced Sensitivity Flex Set reagents. We do not recommend plexing Enhanced Sensitivity Flex Set assays along with assays from any other Flex Set system (soluble protein, cell signaling, etc.). For an updated assay compatibility chart for the BD CBA Human Enhanced Sensitivity Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup.

Performance

Limit of Detection: The theoretical limit of detection is 14.84 fg/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 fg/mL, n=30) + 2 standard deviations.

Product Notices

  1. ProClin is a trademark of Rohm and Haas Company.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Warning: CBA lyophilized standard contains 0.02% (w/w) and Detection Reagent (Part A) contains 0.002% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
561515 Rev. 3
Components
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Description Quantity/Size Part Number
Human IFN-γ Enhanced Sensitivity Capture Bead B8 100 Tests (1 ea) 51-9007207
Human IFN-γ Detection Reagent (Part A) N/A 51-9007208
Human IFN-γ Standard 1 Vial(s) (2 ea) 51-9003507
561515 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.