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      Alexa Fluor® 647 Mouse anti-Human CD192 (CCR2)
      Alexa Fluor® 647 Mouse anti-Human CD192 (CCR2)
      Flow cytometric analysis of Alexa Fluor® 647-conjugated anti-human CCR2 on human peripheral monocytes. Human peripheral leukocytes were stained with Alexa Fluor® 647-conjugated anti-human CCR2 antibody (Clone 48607, Cat. No. 557913; open histogram) or Alexa Fluor® 647-conjugated mouse IgG2b (Clone 27-35, Cat. No. 557903; shaded histogram).  Flow cytometry was performed and the histograms were derived from the gated events based on light scattering characteristics of viable monocytes.
      Alexa Fluor® 647 Mouse anti-Human CD192 (CCR2)
      Flow cytometric analysis of Alexa Fluor® 647-conjugated anti-human CCR2 on human peripheral monocytes. Human peripheral leukocytes were stained with Alexa Fluor® 647-conjugated anti-human CCR2 antibody (Clone 48607, Cat. No. 557913; open histogram) or Alexa Fluor® 647-conjugated mouse IgG2b (Clone 27-35, Cat. No. 557903; shaded histogram).  Flow cytometry was performed and the histograms were derived from the gated events based on light scattering characteristics of viable monocytes.
      Product Details
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      BD Pharmingen™
      CCR2; MCP-1-R; CC-CKR-2; CMKBR2; CKR2; CCR2A; CCR2B; CKR2A; CKR2B
      Mouse IgG2b
      Human hCCR2-transfected NS0 Cells
      Flow cytometry (Routinely Tested)
      20 μl
      VIII 80129
      AB_647150
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      558406 Rev. 1
      Antibody Details
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      48607

      The 48607 monoclonal antibody specifically binds to CD192 which is also known as, Chemokine C-C motif receptor 2 (CCR2), Monocyte chemoattractant protein 1 receptor (MCP-1 receptor/MCP-1-R), or CKR2. CD192 is a seven-transmembrane, G-protein-coupled receptor that is the specific receptor for Monocyte chemoattractant protein 1 (MCP-1/CCL2), MCP-2/CCL8, MCP-3/CCL7, and MCP-4/CCL13. CD192 is expressed on monocytes, dendritic cells, NK cells, basophils, and activated T cells and B cells. CD192 exists in two forms, CD192A and CD192B. The two forms are derived from alternatively spliced variants of a single gene and differ at the C-terminal end. CD192 plays an important role in inflammatory responses.

      558406 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      558406 Rev.1
      Citations & References
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      View product citations for antibody "558406" on CiteAb

      Development References (3)

      1. Charo IF, Myers SJ, Herman A, Franci C, Connolly AJ, Coughlin SR. Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails. Proc Natl Acad Sci U S A. 1994; 91(7):2752-2756. (Biology). View Reference
      2. Wong LM, Myers SJ, Tsou CL, Gosling J, Arai H, Charo IF. Organization and Differential Expression of the Human Monocyte Chemoattractant Protein 1Receptor Gene. EVIDENCE FOR THE ROLE OF THE CARBOXYL-TERMINAL TAIL IN RECEPTOR TRAFFICKING . J Biol Chem. 1997; 272(2):1038-1045. (Biology). View Reference
      3. Yamagami S, Tokuda Y, Ishii K, Tanaka H, Endo N. cDNA cloning and functional expression of a human monocyte chemoattractant protein 1 receptor. Biochem Biophys Res Commun. 1994; 202(2):1156-1162. (Biology). View Reference
      558406 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.